Study on the mechanism of hirudin on diabetes nephropathy model mice
Objective To investigate the therapeutic effect and mechanism of hirudin on diabetes nephropathy in mice.Methods Diabetic nephropathy mouse model was established by high-fat diet and intraperitoneal injection of streptozotocin,and the model mice were randomly divided into model group and hirudin treatment group.Mice in the treatment group were given hirudin by gavage for 8 weeks.The changes of renal function index,urine index,inflammatory factors and inflammatory protein expression in mice were observed.Results After modeling in mice,blood glucose levels significantly increased and significant kidney damage occurred,indicating successful modeling.After hirudin treatment,the levels of blood urea nitrogen(BUN),serum creatinine(CREA)and urinary albumin to creatinine ratio(UACR)decreased significantly,the levels of urine β2-microglobulin(β2-MG)and urinary creatinine(UCr)improved significantly,and the concentrations of inflammatory factors interleukin-1β(IL-1β),interleukin-6(IL-6)and MCP-1 in renal cortex decreased significantly.Further western blot experiments showed that hirudin could significantly inhibit the expression levels of NLRP3 and TLR4 proteins.Conclusion Hirudin has therapeutic effect on diabetic nephropathy mice,and its mechanism may be related to anti-inflammatory effect.
万方数据
维普
