Effect of high-fat diet on lipid and glucose metabolism of rats with malnutrition in infancy of life
Objective To explore the clinical significance of malnutrition in infancy of life on adult life based on LXR-α/SREBP-1c signaling pathway.Methods A total of 32 3-week-old male rats were randomly divided into four groups:the general diet group(group A),the hunger-general diet group(group B),the high-fat group(group C)and the hunger-high-fat group(group D).Fasting blood glucose,triglyceride,homeostasis model assessment of insulin resistance(HOMA-IR)and pathological changes of liver were measured after 0 and 9 weeks of observation.The mRNA expression of liver X receptor-α(LXR-α),sterol Regulatory element binding protein-1c(SREBP-1c),fatty acid synthase(FAS),stearoyl-CoA desaturase 1(SCD1)and peroxisome proliferator-activated receptor-α(PPAR-α)in liver tissue was detected by RT-PCR.Results The insulin levels in groups A,B,C and D were(13.72±0.84),(13.93±1.51),(28.89±3.14)and(32.41±2.25)ng/ml respectively after 9 weeks of dietary feeding,and the insulin levels in groups C and D were higher than those in groups A and B respectively,with statistically significant differences(P＜0.05).At the end of the experiment,the HOMA-IR of groups A,B,C and D were(2.91±0.32),(3.08±0.40),(6.38±0.26)and(7.39±0.48)respectively.Groups C and D were higher than Groups A and B respectively,with statistically significant differences(P＜0.05).In group D,there were lipid-containing vacuoles in hepatocytes,and the central and peripheral areas of lobules were obviously involved.Some rats in groups B and C had mild steatosis.The mRNA expression levels of LXR-α,SREBP-1c,FAS and SCD1 mRNA in the liver of rats in groups C and D were significantly higher than those in groups A and B,with statistically significant differences(P＜0.05).Conclusion The"secondary attack"to rats may lead to the enhancement of lipid synthesis in adult life,which may lead to metabolic abnormalities.
Infancy of lifeMalnutritionLipid metabolismSignaling pathway
万方数据
维普
