Research on the screening of active fractions of Equisetum arvense L.for hemostasis and its function mechanism
Objective To screen the active fractions of Equisetum arvense L.for hemostasis,so as to investigate its hemostatic mechanism.Methods The extracts of petroleum ether,ethyl acetate,n-butanol and water fractions of Equisetum arvense L.were prepared,and Kunming mice were randomly divided into 14 groups:the blank group,the Yunnan Baiyao Powder group,the water fraction group,the n-butanol fraction group,the ethyl acetate fraction group and the petroleum ether fraction group(dosage of each polar fraction:6,4 and 2 g/kg).After 7 days of continuous administration,the bleeding time(BT)and coagulation time(CT)were measured by tail-cutting method and orbital blood collection method.Four coagulation parameters were measured using an automatic coagulation analyzer,which were prothrombin time(PT),thrombin time(TT),activated partial thromboplastin time(APTT)and fibrinogen(FIB)concentration.Results Compared with the blank group,the ethyl acetate fraction(dosages:6,4,2 g/kg)group and the petroleum ether fraction(dosage:6 g/kg)group could remarkably shorten the BT,CT,PT and APTT of mice(P＜0.01).The petroleum ether fraction(dosage:4 g/kg)group could shorten the BT,CT,PT and APTT of mice(P＜0.05).The n-butanol fraction(dosages:6,4,2 g/kg)group,the water fraction(dosages:6,4,2 g/kg)group,and the petroleum ether fraction(dosage:2 g/kg)group had no remarkable impacts on the BT,CT,PT,and APTT of mice(P＞0.05).Each extraction fraction had no remarkable impact on the TT and FIB concentrations in mice(P＞0.05).Conclusion The active fractions of Equisetum arvense L.for hemostasis are petroleum ether fraction and ethyl acetate fraction,and the function mechanism is related to the activation of endogenous and exogenous coagulation systems.
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