Analysis on the difference of components of water extracts of tortoise shell of yellow pond turtle at different decoction time based on widely targeted metabolomics
Objective To analyze the change situation of water extracts of tortoise shell of yellow pond turtle at different decoction time.Methods A total of 9 samples in this experiment were selected and divided into 3 groups for metabolic study.Firstly,the mixed samples were detected by non-targeted metabolomics technology,and the water extracts in the samples were identified.Secondly,these water extracts were combined with the self-built database,and thirdly,the metabolic analysis was carried out based on Targeted Metabolome(TM)widely targeted metabolomics technology.The integrated multivariate statistical analysis,such as the detection platform based on liquid chromatography quadrupole-time-of-flight mass spectrometry(LC/QTOF-MS/MS),the self-built database and repeated correlation evaluation,principal component analysis and orthogonal partial least squares discriminant analysis were used to study the metabolic differences between samples.Results A total of 1248 water extracts were identified,in 8 categories(amino acids and their metabolites,heterocyclic compounds,fatty acyl,glycerol phospholipids,aldehydes,ketones,esters,coenzymes and vitamins,tryptamine,choline,pigments,etc.),among which 113 different water extracts were screened for A vs.B.85 different water extracts were screened by B vs.C,187 different water extracts were screened by A vs.C.The relative content of differential water extract was the most abundant in the final cooking stage.Conclusion In this study,the differences and characteristic compounds of the metabolites of tortoise shell at three different decoction time were preliminarily explained from the perspective of TM widely targeted metabolomics.In view of the close relationship between the metabolites of yellow pond turtle and people's health and nutrition,it can provide reference information and theoretical basis for the development and application of deep-processed products of tortoise shell of yellow pond turtle.
Tortoise shell of yellow pond turtleWater extractsMultivariate statistical analysisUltra high performance liquid chromatography/quadrupole time-of-flight mass spectrometer
万方数据
维普
