Application of cell wax block combined with immunohistochemistry in the diagnosis of serous cavity effusion
Objective To investigate the application of cell wax block combined with immunohistochemistry in pathological diagnosis of serous cavity effusion.Methods A total of 81 cases of suspected malignant serous cavity effusion admitted to Zhongxiang People's Hospital from June 2022 to June 2023 were analyzed retrospectively.All samples were examined by conventional cytology smear and cell wax block combined with immunohistochemistry.The pathological diagnosis of surgery or biopsy was the gold standard,and the diagnostic results and diagnostic efficiency of the two examination methods were compared and analyzed.The microscopic characteristics of the two examination methods were observed,and the tumor source of malignant serous cavity effusion was judged by combining with immunohistochemistry.Results Of the 81 cases of serous cavity effusion,70 cases were diagnosed as malignant by surgery or biopsy.The diagnostic sensitivity,specificity and accuracy of conventional cytology smear were 52.9％,54.5％and 51.9％,respectively.The diagnostic sensitivity,specificity and accuracy of cell wax block combined with immunohistochemistry were 95.7％,27.3％and 92.6％,respectively.The diagnostic sensitivity and accuracy of cell wax block combined with immunohistochemistry were obviously higher than those of conventional cytology smear,with statistically significant differences(P＜0.05).A total of 67 positive cases were detected by cell wax block combined with immunohistochemistry,including 47 cases of lung adenocarcinoma,4 cases of breast cancer,5 cases of ovarian cancer,6 cases of digestive tract origin,2 cases of thyroid cancer,1 case of small cell lung cancer and 2 cases of malignant mesothelioma.Conclusion The combination of cell wax block and immunohistochemistry can improve the diagnostic accuracy of serous cavity effusion cells,and the source of tumor tissue can be clearly determined by immunohistochemistry.
万方数据
维普
