Research on the impact of cardamonin on sperm macrophages and anti-sperm antibodies
Objective To study the impact of sperm macrophages and anti-sperm antibodies positive on sperm quality,and to investigate whether cardamonin can protect sperm by acting on sperm macrophages and anti-sperm antibodies.Methods High-quality sperm was obtained by Percoll gradient centrifugation,and it was co-cultured with macrophages of different concentrations to screen out the concentration range of macrophages that were harmful to sperm.Then,macrophages and sperm suspensions with positive anti-sperm antibodies containing the damage concentration range were co-cultured with a certain amount of cardamonin separately and their combination,and the results were observed.Results The survival time of sperm in the sperm suspension group(the high concentration group)with macrophages and the concentration of 0.6×106/ml or above was significantly shorter than that in the blank group(the sperm suspension group).Compared with the blank group,the survival time of sperm in the anti-sperm antibodies positive sperm suspension group containing high concentration macrophages was significantly reduced,with statistically significant difference(P<0.01).In addition,cardamonin had a protective effect on macrophages,anti-sperm antibodies and their combination in sperm damage,with statistically significant differences on improving sperm survival rate(P<0.01),and with statistically significant differences in reducing sperm DNA fragmentation rate and deformity rate(P<0.05).Conclusion A certain concentration of macrophages,anti-sperm antibodies and their coexistence can significantly reduce the number of sperm survival.The preliminary study shows that cardamonin can reverse the damage of macrophages and anti-sperm antibodies to sperm.
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