Experimental study on electroacupuncture promoting autophagy in a mouse model of Parkinson's disease by regulating the Notch signaling pathway
Objective To observe the protective effect of electroacupuncture on Parkinson's disease(PD)mice and explore its mechanism.Methods A total of 36 C57BL/6 male mice were randomly divided into four groups:control group,Parkinson's disease group(PD group),electroacupuncture group and electroacupuncture+Jagged1(Notch agonist)group.The behavioral indicators and the number of tyrosine hydroxylase(TH)positive neurons in the substantia nigra of the four groups of mice were compared,and the levels of Notch1,p62 and α-synuclein(α-Syn)in striatum were measured.Results The steering time and total climbing time of the electroacupuncture group were lower than those of the PD group,and the step length and TH staining positive cell count were higher than those of the PD group(all P<0.05).The steering time and total climbing time of the electroacupuncture+Jagged1 group were higher than those of the electroacupuncture group,and the step length TH staining positive cell count were lower than those of the PD group(all P<0.05).The expression of Notch1,p62 and α-syn in electroacupuncture group were lower than that in PD group(all P<0.05),and Notch1,p62 and α-syn in electroacupuncture+Jagged1 group were higher than those of the electroacupuncture group(all P<0.05).Conclusion Electroacupuncture treatment can improve motor function in Parkinson's disease mice,and its mechanism may be related to inhibiting the Notch signaling pathway and promoting autophagy.
万方数据
维普
