The value of nucleic acid detection of respiratory pathogens in Luoding area in the diagnosis of respiratory diseases in children
Objective To explore the epidemic characteristics of pathogens of acute respiratory infection in children in Luoding area of Guangdong province in recent years,and to provide guiding data for the prevention and treatment of respiratory infection in children and the rational use of antibiotics.Methods From July 2021 to June 2023,1603 children with acute respiratory tract infection in Luoding Peaple's Hospital were selected,and throat/nasopharyngeal swab samples were collected.Fifteen common respiratory tract pathogens were detected by multiplex amplification nucleic acid detection method,and the distribution of various pathogens in children at different ages and seasons was analyzed.Results The total positive rate of detected pathogens was 74.6％(1196/1603),with a single infection rate of 35.4％and a mixed infection rate of 39.2％.Different age groups:The positive detection rates in descending order are young children group(30.3％),infants group(21.8％),preschool children group(17.6％),and primary school children group(5.0％).The positive rates among the groups were compared with each other,and the difference was statistically significant(P＜0.05).Different seasons:The positive detection rate was highest in summer and spring(25.3％and 20.5％respectively),and the infection situation varied in each season.The positive rates in each season were compared with each other,and the difference was statistically significant(P＜0.05).The pathogens prevalent in different seasons were different.In spring,Moraxelle catarrhalis(MC),rhinovirus(HRV)and haemophilus influenzae(Hi)predominated.Respiratory syncytial virus(RSV)and cytomegalovirus(CMV)were prevalent in summer.RSV and MC were prevalent in autumn.MC and influenza virus type B(INF-B)were the most prevalent in winter.Conclusion Respiratory infections in children are sporadic throughout the year in this region,with MC,CMV,and RSV infections being the most common,with summer and spring being the high incidence seasons.It is worth noting in clinical diagnosis and treatment that young people are more prone to developing the disease,and mixed infections account for a higher proportion in the younger age group.Through nucleic acid testing,the pathogen of the patient can be quickly and accurately identified,providing reference for clinical diagnosis and treatment.
万方数据
维普
