Analysis of differences between the combined decoction of Gualou and Xiebai and the single decoction mixture based on GC-MS and network pharmacology
Objective To compare the differences in chemical composition and activity between the combined decoction of Gualou and Xiebai and the single decoction mixture.Methods Gas chromatography-mass spectrometry(GC-MS)was used to detect the components of the sample solution,and network pharmacology and molecular docking were combined to explore the mechanism of intervention in coronary heart disease.Results The chemical composition profiles of the combined decoction and the single decoction mixture were generally similar,but the content of 10 compounds such as pyridine borane,butyric acid,hexanoic acid,and heptanoic acid in the combined decoction was significantly higher than that in the single decoction mixture.The network pharmacology results showed that core components such as n-hexanoic acid,3-benzoyloxypyridine,n-valeric acid,and n-heptanoic acid acted on core targets such as EGFR,VEGFA,STAT3,PPARG,PPARA,and NOS3,affecting HIF-1,PI3K-Akt,Ras,and other signaling pathways to participate in the treatment of coronary heart disease.Conclusion There are differences in the content of some active ingredients related to the treatment of coronary heart disease between the combined decoction of Gualou Xiebai and the single decoction mixture,which provides a theoretical basis for the rational use and further development of Gualou Xiebai Decoction.
万方数据
维普
