摘要
目的 探讨葛花治疗酒精性肝炎(AH)的作用机制.方法 收集葛花活性成分、作用靶点及AH相关靶点,对交集靶点进行蛋白质相互作用分析并获取关键核心靶点,进行功能富集分析,构建葛花成分-交集靶点-疾病-通路网络,并进行分子对接.结果 获得17种葛花主要成分及其靶点166个,AH相关靶点699个,葛花与AH的交集靶点70个;葛花的主要活性成分是槲皮素、山柰酚、葛花苷元、芒柄花黄素及β-谷甾醇,关键核心靶点为丝氨酸/苏氨酸蛋白激酶(AKT1)、肿瘤坏死因子(TNF)、白细胞介素(IL)-6、肿瘤蛋白p53、IL1B;主要信号通路为TNF、IL-17和丝裂原活化蛋白激酶.结论 葛花可能通过槲皮素等活性成分激活AKT1、TNF蛋白,调控TNF等信号通路治疗AH.
Abstract
Objective To explore the mechanism of Ge Hua in the treatment of alcoholic hepatitis(AH).Methods The active components,action targets and AH-related targets of Ge Hua were collected,and the key core targets were obtained,and protein-protein interaction analysis was conducted on the intersection targets,and the function enrichment analysis was carried out,so as to construct a network of Ge Hua components,intersection targets,diseases and pathways,and carry out molecular docking.Results 166 main components and targets of 17 types of Ge Hua were obtained,699 AH-related targets were identified,and 70 intersection targets between Ge Hua and AH were identified.The main active components of Ge Hua were quercetin,kaempferol,puerarin,and mangiferin β-Sitosterol,with serine/threonine-protein kinase 1(AKT1),tumor necrosis factor(TNF),interleukin(IL)-6,tumor protein p53,IL1B as the key core targets.TNF,IL-17,and mitogen-activated protein kinase were the main signaling pathways.Conclusion Ge Hua may activate AKT1 and TNF proteins through active components such as quercetin,and regulate TNF signaling pathways to treat AH.
维普
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