Study on extraction and antibacterial activity of flavonoids from different parts of Tongling ginger by response surface optimization design
Objective To optimize the extraction process of flavonoids from different parts of root,stem and leaf of Tongling ginger and compare their antibacterial activities.Methods Ultrasonic-assisted extraction was used to optimize the extraction process of total flavonoids from ginger by Box-Behnken response surface experiment with the extraction rate of flavonoids as the index and the material to liquid ratio,ultrasonic time and ethanol concentration as the factors.Results The optimum extraction process of flavonoids from ginger was as follows:the material to liquid ratio was 13∶1,the ultrasonic time was 42 minutes,and the ethanol concentration was 76％.The order of flavonoid content in different parts of ginger was root＞leaf＞stem,and the inhibitory effect on Staphylococcus aureus,Pseudomonas aeruginosa and Bacillus subtilis was root＞leaf＞stem.Conclusion The optimized extraction process can be used for the extraction of total flavonoids from different parts of ginger,and the antibacterial test results have reference value for the development of new uses of Tongling ginger.
Tongling gingerFlavonoidsAntibacterial activityExtraction process
万方数据
维普
