Interventional effect of Astragalus polysaccharides on papillary thyroid carcinoma cells miRNA26
Objective To analyze the effect of astragalus polysaccharides (APS) on the expression of microRNA (miRNA) 26 and its target genes in papillary thyroid carcinoma (PTC) cells. Methods PTC TPC-1 cells were routinely cultured in vitro and divided into TPC-1 group (blank) and APS100,APS200 and APS400 groups (treated with 100,200 and 400 μg/ml APS). Inverted microscope was used to observe the cell morphology,CCK-8 assay was used to detect the activity of TPC-1 cells in each group,RT-qPCR assay was used to detect the relative expression of miRNA26 in TPC-1 cells and the intervention effect of APS,RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of FOXO1 and the intervention effect of APS. Results Different doses of APS inhibited the proliferation of TPC-1 cells for 24 h,with the best inhibition effect at a concentration of 200 μg/ml. APS down-regulated the expression of miRNA26 and up-regulated the mRNA and protein expression levels of FOXO1. Conclusion APS may play an inhibitory role on TPC-1 cells by down-regulating miRNA26 and then negatively regulating FOXO1 gene expression.
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维普
