Impact of metformin on inflammatory response in mice with pulmonary fibrosis model
Objective To investigate the anti-inflammatory effect of metformin on bleomycin hydrochloride-induced pulmonary fibrosis model mice. Methods A total of 48 male C57BL/6J mice (no specific pathogen grade),6 to 8 weeks of age and weighing 23-25 g,were divided into the control group,the model group,the pirfenidone group,the metformin group,the pirfenidone combined with metformin group and the reduced nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) activity inhibitor (DPI) group of 8 mice each according to the random number table method. Using bleomycin hydrochloride-induced pulmonary fibrosis in mice as a model,the pathological changes of lung tissue of each group of mice were observed and the levels of tumor necrosis factor-α (TNF-α) were detected. Results HE and Masson staining of lung tissue showed that bleomycin hydrochloride-induced obvious pulmonary fibrosis in mice. The Ashcroft scores of the model group,pirfenidone group,metformin group,pirfenidone combined with metformin group,and DPI group were higher than those of the control group,and the differences were statistically significant (P<0.05). There was a tendency for the lung fibrosis of the mice in the pirfenidone combined with metformin group to be attenuated compared with that of the model group (P<0.05). Metformin could alleviate bleomycin hydrochloride-induced pulmonary fibrosis in mice. When combined with pirfenidone,the level of TNF-α in mice was further reduced compared with single drug intervention. Conclusion The anti-inflammatory effect of metformin combined with pirfenidone is enhanced,which can play a role in the occurrence of pulmonary fibrosis in mice and has a certain role in delaying the progress of pulmonary fibrosis.
万方数据
维普
