Content determination of aristolochic acid I in Xiaozhong Zhitong Tincture by LC-MS/MS method
Objective To establish a specific detection method for aristolochic acid I in Xiaozhong Zhitong Tincture,so as to provide theoretical basis for quality control of Xiaozhong Zhitong Tincture.Methods The content determination of aristolochic acid Ⅰ in Xiaozhong Zhitong Tincture was carried out by liquid chromatography-mass spectrometry(LC-MS/MS).Separation was carried out on a Poroshell 120 SB-C18(2.1 mm × 100 mm,2.7 μ m)column,gradient elution was carried out with 0.1％formic acid solution(containing 5 mmol/L ammonium formate)-acetonitrile as the mobile phase,the column temperature was 30 ℃,and the flow rate was 0.3 ml/min.In the positive ion mode of electrospray ionization,multiple reaction monitoring was carried out,and external standard method was used for quantification.Results The linear relationship of aristolochic acid I was good in the range of 0.1-40 ng/ml(correlation coefficient r=0.9999),the average recovery rate was 114.0％(RSSD=8.7％),and the repeatability and precision of the method were both good.Conclusion The method is simple and can be used to carry out the content determination of aristolochic acid I in Xiaozhong Zhitong Tincture.
万方数据
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