Study on the protective mechanism of hyperoside on methamphetamine-induced PC 12 cell injury
Objective To explore the effect of hyperoside on methamphetamine-induced nerve cell injury and its possible mechanism.Methods A methamphetamine-induced nerve cell injury model was established,and cell survival rate was detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide method,deoxyribonucleic acid(DNA)damage was detected using comet electrophoresis.Oxidative stress-related indicators in nerve cells and KELCH-like ECH associated protein 1(Keap1)/nuclear factor E2 associated factor 2(Nrf2)pathway protein expression changes were detected,and the possible mechanism of the protective effect of hyperoside was further explored.Results Compared with the control group,the survival rate and antioxidant capacity of cells in methamphetamine group decreased significantly,while the degree of DNA damage and apoptosis increased,with statistically significant difference(P＜0.05).Compared with the methamphetamine group,the antioxidant capacity,Nrf2 levels in nucleus and the expressions of its downstream proteins heme oxygenase-1 and γ-glutamylcysteine synthetase were significantly higher,and the degree of apoptosis and DNA damage was significantly decreased in the hyperoside intervention group(P＜0.05).Conclusion Hyperoside can reduce methamphetamine-induced nerve cell injury,and its protective mechanism may be related to activating Keap1/Nrf2 pathway and promoting nuclear translocation of Nrf2 and its downstream protein expression.
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