Extraction of Antarctic krill oil by enzymatic hydrolysis
Extraction of Antarctic krill oil by enzymatic hydrolysis combining with solvent extraction was studied. According to comparative experiments, the best method was adding Alcalase firstly and then adding Neutrase, and the influences of dosages and enzymatic time of the two enzymes on extraction rate of krill oil were studied. The optimal extraction conditions were determined by single factor experiment and orthogonal experiment as follows: ratio of material to liquid 1:3.4, Alcalase dosage 0. 1 % , pH 8, temperature 55 ℃ ,time 2 h, Neutrase dosage 0. 1% ,pH 7, temperature 50℃ ,time 2. 5 h. Under the optimal conditions the extraction rate of krill oil was up to 64. 52%. The physicochemical indexes of the krill oil were as follows;acid value 61.07 mgKOH/g,iodine value 157.87 gI/100 g,peroxide value 0.45 mmol/kg, water and volatile matter 0. 84% ,unsaponifiable matter 0.64% ,unsolvable impurity 0. 09% . Unsaturated fatty acids content reached 71.24% in the krill oil,in which the main components were EPA(9. 82% ) and DHA(21.97% ) ,and the oil had high nutritional value.
NETL
NSTL
万方数据
维普
