Aqueous extraction of tea seed oil from tea seed kernel was investigated. The effects of raw material particle size, extraction temperature, ratio of water to material and extraction time on extraction rate of tea seed oil were discussed. The optimal conditions of aqueous extraction of tea seed oil were determined by orthogonal experiment as follows: raw material particle size 60 - 80 meshes, extraction temperature 90℃, ratio of water to material 3.5:1,extraction time 3 h. Under these conditions, extraction rate of tea seed oil was 90.2%.