Hydrolysis of heat-denaturized soy protein isolate with Alcalase or Papain
The heat-denaturized soy protein isolate was hydrolyzed by Alcalase and Papain respectively, and the effects of enzymolysis temperatures of two kinds of proteases on the enzymolysis result of soy pro-tein isolate were investigated. The results showed that the enzymolysis of soy protein isolate with Alcalase at 60℃ was deeper than that at 70℃, more low relative molecular weight peptides were obtained and the degree of hydrolysis of soy protein isolate was 14.73%;while temperature had little effect on the hydroly-sis of soy protein isolate with Papain;more small peptides could be obtained by the hydrolysis of soy pro-tein isolate with Alcalase than that with Papain.