Antioxidant activities of peony seed shell extract and different polarity fractions
The peony seed shell extract(70％ ethanol solution extract) was separated step by step by different polarity solvents (petroleum ether,ethyl acetate,n-butanol and water) to obtain different polarity fractions extracts.The contents of total polyphenol in peony seed shell extracts and each polarity fraction extract were researched,and the antioxidant activities were investigated by DPPH and FRAP methods with L-ascorbic acid (Vc) and 2,6-di-tert-butyl-4-methylphenol (BHT) as reference antioxidants.The results showed that the contents of total polyphenol in different extracts varied greatly,and the sequence from low to high was:water extract,n-butyl extract,ethyl acetate extract and peony seed shell extract,in the range of 1.30-57.50 mg/g.The peony seed shell extract and different polarity fractions extracts had certain antioxidant capacity,the antioxidant activity of n-butanol extract was the lowest,followed by water extract,peony seed shell extract and ethyl acetate extract.The range of FRAP value was 27.28-1 250.08 mtol/L,in which FRAP values of VC and BHT were 1 814.88,226.00 mtol/L,respectively.The scavenging activity of peony seed shell extract on DPPH free radical was the lowest,followed by ethyl acetate extract,n-butanol extract and water extract,and the range of IC50 was 22.47-71.00 μg/mL,in which the IC50 of BHT was 34.30 μg/mL.
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