Optimization of extraction of seabuckthorn fruit oil by response surface methodology
With seabuckthorn pulp as raw material,seabuckthorn fruit oil was extracted by compound enzymatic hydrolysis technology.The effects of enzyme dosage,mass ratio of pectinase to cellulose,hydrolysis temperature and hydrolysis time on the yield of seabuckthorn fruit oil were studied.Based on single factor experiment,the extraction conditions were optimized by Box-Behnken response surface methodology as follows:enzyme dosage 0.30 g(based on 100 g seabuckthorn pulp),mass ratio of pectinase to cellulase 1.54∶1,hydrolysis temperature 55 ℃ and hydrolysis time 4.24 h.Under these conditions,the yield of seabuckthorn fruit oil was 5.28％,increasing by 19.7％ than that before optimization.
seabuckthorn fruit oilextractionresponse surface methodology
NETL
NSTL
万方数据
维普
