首页|超临界CO2法萃取树莓籽油的成分分析

超临界CO2法萃取树莓籽油的成分分析

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采用超临界CO2技术萃取树莓籽油,利用GC法测定脂肪酸组成,GC-MS联用法测定甾醇组成,HPLC测定生育酚含量,紫外可见分光光度计测定多酚含量.结果表明:超临界CO2萃取树莓籽油的平均出油率为17.90%±0.3%;共检测到10种脂肪酸,其中亚油酸相对含量(57.44%)最高,其次是α-亚麻酸(26.58%)、油酸(11.94%)和棕榈酸(2.10%);脂肪伴随物中,共检测出7种甾醇,总甾醇含量为861.85 mg/100 g;γ-生育酚、α-生育酚和 δ-生育酚含量分别达到799.248、602.996 mg/kg及13.371 mg/kg;多酚平均含量为(341.19 ±8.94)mgGAE/kg.此研究为树莓籽油及其他蔷薇科植物油的进一步开发利用奠定了基础.
Component analysis of raspberry seed oil extracted by supercritical CO2
The supercritical CO2 was used to extract the raspberry seed oil,the fatty acid composition was determined by GC,the sterol composition was determined by GC -MS,the content of tocopherol was determined by HPLC,and the polyphenol content was determined by UV visible spectrophotometer.The results showed that the extraction of raspberry seed oil by supercritical CO2 was more efficient and simple, with an average oil yield of 1 7.90%±0.3%.A total of ten fatty acids were detected,of which the rela-tive content of linoleic acid(57.44%)was the highest,followed by α-linolenic acid(26.58%),oleic acid(1 1.94%)and palmitic acid(2.1 0%).Seven kinds of sterols were detected,and the total content of sterols was 861.85 mg/1 00 g.In addition,the contents of γ-tocopherol,α-tocopherol and δ-to-copherol were 799.248,602.996 mg/kg and 1 3.371 mg/kg,respectively.The average content of poly-phenols was(341.1 9 ±8.94)mgGAE/kg.It laid the foundation for the further development and utilization of raspberry seed oil and other Rosaceae vegetable oils.

supercritical CO2extractionraspberry seed oilfatty acidsteroltocopherolpolyphenol

刘雅萍、李鸿雁、郑莉、常明、李斌、金青哲、王兴国

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江南大学 食品学院,江苏 无锡214122

沈阳农业大学 食品学院,沈阳110866

超临界CO2萃取 树莓籽油 脂肪酸 甾醇 生育酚 多酚

江苏高校品牌专业建设工程资助项目

PPZY2015A052

2017

中国油脂
国家粮食储备局西安油脂科学研究设计院

中国油脂

CSTPCDCSCD北大核心
影响因子:0.842
ISSN:1003-7969
年,卷(期):2017.42(12)
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