Optimization of preparation of astaxanthin nanolipid carrier based on central composite design method
In order to improve the bioavailability and increase the stability of astaxanthin,astaxanthin nanolipid carrier(AST-NLC)was prepared by the homogeneous emulsification-probe ultrasonic method.The preparation process of AST-NLC was optimized by single factor experiment and central composite design method with particle size and encapsulation rate as indexes,and the AST-NLC prepared was structurally characterized by X-ray diffraction(XRD)and Fourier infrared spectroscopy(FT-IR)and tested for thermal stability at 4,25 ℃ and 50℃.The results showed that the optimal process conditions for preparing AST-NLC were mass ratio of glycerol tristearate to medium chain triglyceride 1∶11(total mass 0.99 g),emulsifier dosage(Tween-80+soybean lecithin,mass ratio 1∶1)3.8％(based on water phase mass),oil-water volume ratio 1∶34,homogeneous speed 5 000 r/min,ultrasonic time 2 min,astaxanthin dosage 2.0 mg.Under the optimal conditions,the AST-NLC was prepared with a particle size of(79.30±1.21)nm,a polymer dispersity index(PDI)of 0.01±0.01,an encapsulation rate of(81.36±1.84)％,and Zeta potential(-18.13±2.83)mV.The structural analysis revealed that astaxanthin in AST-NLC was encapsulated in liposomes in the state of molecules.The thermal stability results showed 4 ℃ was more favorable for the preservation of AST-NLC.In conclusion,AST-NLC with small particle size,homogenous dispersion and high encapsulation rate was successfully prepared.
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