Optimization of debittering of sesame ACE inhibitory peptide by activated carbon and resin and their performance comparison
To improve the taste of sesame ACE inhibitory peptide,activated carbon and resin were used to eliminate the bitterness of sesame ACE inhibitory peptide,and the debittering rate and peptide loss rate were taken as the evaluation indexes,single factor experiment and orthogonal experiment were used to optimize the two debittering process conditions.The suitable debittering method was obtained based on the comparison in taste characterization,sesame ACE inhibitory peptide activity,and amino acid composition between the two debittering methods.The results showed that both methods could reduce the bitterness of sesame ACE inhibitory peptide to imperceptible.The optimal debittering conditions of activated carbon were as follows:addition of activated carbon No.2 6％,sesame ACE inhibitory peptide solution mass concentration 10 g/100 mL,absorption temperature 55 ℃,absorption time 15 min.Under the optimal conditions,the debittering rate and peptide loss rate were(96.46±0.57)％ and(29.02±0.19)％(bitterness detected by electronic tongue was-0.61±0.02).While the debittering rate and peptide loss rate of AB-8 macroporous resin reached(95.21±0.93)％and(12.11±0.19)％(the bitterness was-0.03± 0.08)under the optimal conditions of AB-8 macroporous resin dosage 12.5％,sesame ACE inhibitory peptide solution mass concentration 10 g/100 mL,pH 7,absorption temperature 35 ℃ and absorption time 15 min.The activity of ACE inhibitory peptide debittered by AB-8 macroporous resin was significantly stronger than that debittered by activated carbon,which was related to the difference in peptide composition caused by the debittering methods.Overall,AB-8 macroporous resin adsorption can better reduce the contents of bitter amino acids,and is suitable for sesame ACE inhibitory peptide debittering.
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