Simultaneous determination of cannabinol,cannabidiol and Δ9-tetrahydrocannabinol in edible vegetable oil by solid-phase extraction purification coupled with ultra performance liquid chromatography-tandem mass spectrometry
In order to effectively improve the risk monitoring level of cannabinoid compounds in edible vegetable oils,a method for the simultaneous determination of cannabinol,cannabidiol and Δ9-tetrahydrocannabinol in edible vegetable oils by solid-phase extraction purification coupled with ultra performance liquid chromatography-tandem mass spectrometry was established,and the accuracy of the method was verified by the limit of detection,limit of quantification,uncertainty,spiked recovery and precision.The results showed that the extracts were extracted twice with 5 mL acetonitrile,purified by an EMR-Lipid-type solid-phase extraction column,eluted with a gradient of water and methanol as the mobile phases,and separated on an Agilent Pursuit 3 PFP(3 μm,2.0 mm × 150 mm)type column,with ionisation in the negative ion mode,monitored by the multiple reaction mode,and quantified by isotopic internal standard method in the matrix standard curve.Under the conditions of this method,cannabinol,cannabidiol and Δ9-tetrahydrocannabinol showed good linearity in the mass concentration range of 5-200 ng/mL,and the limits of detection were all 10 μg/kg,with the spiked recoveries ranging from 84.27％to 100.30％and the relative standard deviations of 1.2％-3.9％;the presence of cannabinoids was detected in the determination of some edible vegetable oils in the actual samples.The method is simple,rapid,accurate and suitable for the determination of cannabinol,cannabidiol and Δ9-tetrahydrocannabinol in edible vegetable oils.
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