首页|响应面法优化芝麻粕发酵制备小肽的工艺研究

响应面法优化芝麻粕发酵制备小肽的工艺研究

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为充分开发并有效利用芝麻粕,以小肽含量为指标,通过Plackett-Burman试验、最陡爬坡试验和响应面试验对米曲霉发酵芝麻粕制备小肽的工艺进行优化。结果表明,米曲霉发酵芝麻粕制备小肽的最佳工艺条件为芝麻粕与麸皮质量比9∶1、米曲霉接种量0。01%、发酵温度31。3℃、发酵时间64。24 h、料水比1∶1。18,在此条件下发酵样品中的小肽含量可达20。04%。通过米曲霉发酵芝麻粕,可得到富含小肽的产品。
Optimization of preparation of small peptides from sesame meal by response surface methodology
In order to fully develop and effectively use sesame meal,the preparation process of small peptide from sesame meal fermented by Aspergillus oryzae was optimized by Plackett-Burman experiment,steepest ascent experiment and response surface methodology,with the content of small peptides as the index.The results showed that the optimal conditions for the preparation of small peptides were obtained as follows:mass ratio of sesame meal to bran 9∶1,inoculation amount of Aspergillus oryzae 0.01%,fermentation temperature 31.3℃,fermentation time 64.24 h,and ratio of material to water 1∶1.18.Under these conditions,the content of small peptides in the fermented samples could be up to 20.04%.Products rich in small peptides can be obtained from sesame meal fermented by Aspergillus oryzae.

sesame mealAspergillus oryzaefermentationsmall peptidesresponse surface methodology

王飞、姜舒、杨蔚薇、罗杨海、彭楠、赵述淼、胡咏梅

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华中农业大学生命科学技术学院,武汉 430070

湖北省普林标准技术服务有限公司,武汉 430056

芝麻粕 米曲霉 发酵 小肽 响应面法

"科技助力经济"重点专项(2020)湖北洪山实验室重大项目湖北省大学生创新创业训练计划

2021hszd022S202110504086

2024

中国油脂
国家粮食储备局西安油脂科学研究设计院

中国油脂

CSTPCD北大核心
影响因子:0.842
ISSN:1003-7969
年,卷(期):2024.49(4)
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