Fabrication of rapeseed protein isolate-carrageenan conjugate-pepper essential oil Pickering emulsion and analysis of its physicochemical properties
In order to promote the development and utilization of pepper essential oil and rapeseed protein,rapeseed protein isolate(RPI)-carrageenan conjugate nanoparticle was prepared by thermal induction method,and Pickering emulsion was prepared to load pepper essential oil from this material.The preparation conditions of RPI-carrageenan conjugate-pepper essential oil Pickering emulsion were optimized by single factor experiment and response surface methodology.The type,microstructure,thermal stability and storage stability of the prepared RPI-carrageenan conjugate-pepper essential oil Pickering emulsion were characterized,and its antibacterial activities were investigated.The results showed that the optimal conditions for the preparation of RPI-carrageenan conjugate-pepper essential oil Pickering emulsion were obtained as follows:dosage of RPI-carrageenan conjugate nanoparticle 0.95％,dosage of pepper essential oil-soybean oil mixture(volume ratio 2:3)50.6％,and aqueous phase pH 9.8.Under these conditions,the encapsulation rate was 92.34％.The prepared RPI-carrageenan conjugate-pepper essential oil Pickering emulsion was oil-in-water type,which had good stability at 20-60 ℃,but was not stable above 60 ℃.After 30 d storage,the average particle size of the emulsion changed little.The emulsions had good milk droplet characteristics,which provided a physical basis for stability.Compared with the blend of pepper essential oil and soybean oil,the RPI-carrageenan conjugate-pepper essential oil Pickering emulsion showed similar antibacterial effect within 42 h of action on the subject bacteria,but showed stronger antibacterial effect after 42 h.In conclusion,RPI-carrageenan conjugate-pepper essential oil Pickering emulsion has higher encapsulation rate and superior thermal stability,storage stability and antibacterial activity.
rapeseed protein isolatecarrageenanPickering emulsionpepper essential oilantibacterial effect
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