Research progress on enzymatic preparation of human milk fat substitutes
Human milk fat substitutes are a kind of structural lipids that simulate the composition of breast milk fat.In order to promote green and efficient production of human milk fat substitutes,the triglyceride composition of breast milk fat was introduced,and the enzymatic preparation technology and practical application of human milk fat substitutes were analyzed,summarized and discussed,and the future research direction was prospected.The content of 1-oleoyl-2-palmitoyl-3-linoleoyl glycerol(OPL)was the highest in Chinese breast milk fat,followed by 1,3-dioleoyl-2-palmitoyl glycerol(OPO).The enzymatic preparation of human milk fat substitutes includes ester-esterification,one-step acid hydrolysis,alcohololysis-esterification two-step,two-step acid hydrolysis,transesterification-acid hydrolysis two-step,etc.Improving the catalytic activity and location selectivity of enzymes and inhibiting acyl transfer are the future research directions of enzymatic preparation of human milk fat substitutes.
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