Preparation and in vitro anti-inflammatory activity of genistein-loaded nanoliposomes
To improve the bioavailability of genistein(Gen)as well as to provide a reference for the development of lipid-soluble bioactive substances in novel functional foods,nanoliposomes loaded with Gen(Gen@Lip)were prepared by film dispersion method using soy lecithin and cholesterol as raw materials,and their basic indexes,structure,storage stability,in vitro release characteristics and anti-inflammatory activity were investigated.The results showed that the Gen loading,average particle size,polydispersity index,and Zeta potential of the prepared Gen@Lip was 36％,(242.73±2.03)nm,0.32 and(-16.33±1.16)mV,respectively.The structural characterization results confirmed that Gen was effectively embedded in Gen@Lip,and Gen@Lip had good storage stability.Gen@Lip was able to efficiently pass through the stomach to enter into the intestines to achieve a slow release effect.Compared with free Gen,Gen@Lip had a stronger anti-inflammatory effect on the Raw 264.7 cells and had no toxic side effects within the range of use(＜100 μmol/L).In conclusion,the Gen@Lip prepared can improve the bioavailability of Gen and has significant anti-inflammatory effects.
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