Protective effect of odd-chain fatty acids on lipopolysaccharide-induced inflammation in Caco-2 cells
In order to explore the health effects of odd-chain fatty acids(OCFA),serum OCFA levels in the healthy individuals and colon cancer patients were measured by gas chromatography-mass spectrometry(GC-MS)to investigate the differences in the distribution characteristics,and the protective effects of OCFA on inflammation by using the lipopolysaccharide(LPS)-induced Caco-2 cell inflammation model were studied,including the changes in cell activity,pro-inflammatory factor gene expression and fatty acid composition.The results showed that the levels of two main OCFA(C15:0 and C17:0)in healthy individuals were significantly higher than that in colon cancer patients.The levels of C15:0 and C17:0 in male patients and patients over the age of 59 were significantly lower.C15:0 and C17:0 had no effect on cell activity.C15:0 could significantly inhibit the expression of pro-inflammatory factors TNF-α,IL-8 and iNOS in Caco-2 cells with LPS stimulation,C17:0 could significantly reduce the expression of IL-6,TNF-α,IL-8 and iNOS.Two main OCFA could play a similar role in alleviating inflammatory stimulation as n-3 PUFA.Two main OCFA could be easily incorporated by Caco-2 cells into C17:0 and C17:1 in Caco-2.In conclusion,OCFA may have a potential preventive effect on inflammation and intestinal health by regulating the expression of pro-inflammatory cytokines.
OCFAC1 5:0C17:0intestinal inflammationintestinal health
万方数据
维普
