Effect of glycosylation on interfacial structure and stabilities of oil body extracted from Camellia oleifera seed
Oil body is the oil storage organelle in oilseeds,which is stabilized by a monolayer of phospholipids and membrane proteins.In order to increase the pH stability of oil body,glucose and lactose were used as carbonyl donors to modify the interfacial structure of oil bodies(OBs)extracted from Camellia oleifera seed via glycosylation.The physical stabilities of OBs were evaluated via particle size and Zeta-potential.The molecular weight and solubility of OBs membrane protein were investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Thermo Scientific Pierce BCA kit,respectively.The interfacial structure of OBs was observed by Raman-confocal microscope.The results showed that the glycosylation reaction increased the particle size of Camellia oleifera OBs,but the particle size became more uniform,and the Zeta-potential did not change significantly(p＞0.05).Under the conditions of mass ratio of glucose to OBs 2∶1,and mass ratio of lactose to OBs 5∶1,the glycosylated OBs membrane protein exhibited the highest solubility,with increase of 26.8 times and 16.1 times compared with the control(unglycosylation),respectively.The molecular weight of OBs membrane protein increased slightly after glycosylation.Raman spectra analysis showed that the glucose and lactose were successfully grafted on membrane proteins of OBs.In conclusion,glycosylation is beneficial for improving the stability of OBs.
Camellia oleifera oil bodyglycosylationinterfacial structureoil body membrane proteinstability
NETL
NSTL
万方数据
