不同加工方式的油茶籽油对变应性接触性皮炎的抗炎效果
Anti-inflammatory effect of oil-tea camellia seed oil extracted by different methods on allergic contact dermatitis
陈宇 1杨纪元 1邱之阳 1张笑 1邱昌扬 1周凯 2郝泽金 3陈志敏4
作者信息
- 1. 九江学院江西油茶研究中心/药学与生命科学学院/九江市食品加工与安全重点实验室,江西九江 332005
- 2. 九江学院江西油茶研究中心/药学与生命科学学院/九江市食品加工与安全重点实验室,江西九江 332005;岭南现代农业科学与技术广东省实验室河源分中心,广东河源 517001
- 3. 江西神州通油茶科技有限公司,江西 九江 332801
- 4. 江西晟启农业科技有限公司,江西 九江 332621
- 折叠
摘要
为了明确不同加工方式的油茶籽油对变应性接触性皮炎(ACD)的治疗效果,并探究油茶籽油中的主要抗炎物质,分别制备低温压榨油茶籽油、热榨油茶籽原油、热榨精炼油茶籽油、土榨油茶籽油和超临界CO2油茶籽油,分析不同加工方式及添加不同活性物质的油茶籽油对ACD模型小鼠耳廓肿胀的影响,并结合ELISA法、HE染色和免疫组化探究其对小鼠炎症的抑制效果.结果表明:超临界CO2油茶籽油对ACD小鼠耳廓肿胀的抑制率最高,与阳性对照组相比其小鼠血清中炎症因子TNF-α、IL-1β和IL-6含量降低率超过10％,炎症细胞浸润程度明显好转,耳廓肿胀组织中NF-κB阳性表达量与阴性对照组相比无显著差异;预防性给药的抗炎效果普遍低于造模后给药;倍数添加角鲨烯和生育酚的油茶籽油对ACD小鼠耳廓肿胀的抑制率显著提高,在50％左右,且角鲨烯、生育酚处理组小鼠耳廓组织中NF-κB阳性表达量与阴性对照组无显著差异.因此,外用超临界CO2萃取的油茶籽油治疗小鼠ACD效果最佳,其抗炎效果并非源于单一活性成分,与角鲨烯和生育酚等活性成分均密切相关.
Abstract
In order to clarify the therapeutic effect of oil-tea camellia seed oil extracted by different methods on allergic contact dermatitis(ACD),and further explore the potential active substances of anti-inflammatory in oil-tea camellia seed oil,cold pressed oil-tea camellia seed oil,hot pressed crude oil-tea camellia seed oil,hot pressed-refined oil-tea camellia seed oil,native pressed oil-tea camellia seed oil,and supercritical CO2 extracted oil-tea camellia seed oil were prepared,the effects of different processing methods of oil-tea camellia seed oils and different active substances added with oil-tea camellia seed oils on auricular swelling in ACD model mice were analyzed,and their anti-inflammatory effect in mice were studied by combining ELISA method,HE staining,and immunohistochemistry.The results showed that the supercritical CO2 extracted oil-tea camellia seed oil showed the highest inhibition rate on auricular swelling of ACD mice,and the inflammatory factors,such as TNF-α,IL-1β and IL-6 were reduced more than 10％compared with positive control.The degree of inflammatory infiltration significantly improved,and there was no significant difference in NF-κB positive expression of auricular tissue between supercritical CO2 extracted oil-tea camellia seed oil treatment group and negative control.However,the anti-inflammatory effect of prophylactic administration was generally lower than that of post modeling administration.The oil-tea camellia seed oil that supplemented with multiple squalene and tocopherol increased the inhibition rate of auricular swelling about 50％.There was no significant difference in NF-KB positive expression of auricular tissue between the squalene and tocopherol group and the negative control.In general,supercritical CO2 extracted oil-tea camellia seed oil exhibits the best therapeutic effect on ACD mice,and the active anti-inflammatory ingredients are closely related to squalene and tocopherol.
关键词
油茶籽油/抗炎/变应性接触性皮炎/超临界CO2萃取Key words
oil-tea camellia seed oil/anti-inflammatory/allergic contact dermatitis/supercritical CO2 extraction引用本文复制引用
基金项目
岭南现代农业科学与技术广东省实验室河源分中心(DT20220005)
江西省卫生健康委科技计划项目(SKJP220201588)
江西省中医药管理局科技计划项目(2020A0373)
江西省教育厅科学技术研究项目(GJJ211804)
江西省林业局油茶专项项目(YCYJZX[2023]333)
出版年
2024
NETL
NSTL
万方数据