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不同加工方式的油茶籽油对变应性接触性皮炎的抗炎效果

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为了明确不同加工方式的油茶籽油对变应性接触性皮炎(ACD)的治疗效果，并探究油茶籽油中的主要抗炎物质，分别制备低温压榨油茶籽油、热榨油茶籽原油、热榨精炼油茶籽油、土榨油茶籽油和超临界CO2油茶籽油，分析不同加工方式及添加不同活性物质的油茶籽油对ACD模型小鼠耳廓肿胀的影响，并结合ELISA法、HE染色和免疫组化探究其对小鼠炎症的抑制效果。结果表明:超临界CO2油茶籽油对ACD小鼠耳廓肿胀的抑制率最高，与阳性对照组相比其小鼠血清中炎症因子TNF-α、IL-1β和IL-6含量降低率超过10％，炎症细胞浸润程度明显好转，耳廓肿胀组织中NF-κB阳性表达量与阴性对照组相比无显著差异;预防性给药的抗炎效果普遍低于造模后给药;倍数添加角鲨烯和生育酚的油茶籽油对ACD小鼠耳廓肿胀的抑制率显著提高，在50％左右，且角鲨烯、生育酚处理组小鼠耳廓组织中NF-κB阳性表达量与阴性对照组无显著差异。因此，外用超临界CO2萃取的油茶籽油治疗小鼠ACD效果最佳，其抗炎效果并非源于单一活性成分，与角鲨烯和生育酚等活性成分均密切相关。
Anti-inflammatory effect of oil-tea camellia seed oil extracted by different methods on allergic contact dermatitis
In order to clarify the therapeutic effect of oil-tea camellia seed oil extracted by different methods on allergic contact dermatitis(ACD),and further explore the potential active substances of anti-inflammatory in oil-tea camellia seed oil,cold pressed oil-tea camellia seed oil,hot pressed crude oil-tea camellia seed oil,hot pressed-refined oil-tea camellia seed oil,native pressed oil-tea camellia seed oil,and supercritical CO2 extracted oil-tea camellia seed oil were prepared,the effects of different processing methods of oil-tea camellia seed oils and different active substances added with oil-tea camellia seed oils on auricular swelling in ACD model mice were analyzed,and their anti-inflammatory effect in mice were studied by combining ELISA method,HE staining,and immunohistochemistry.The results showed that the supercritical CO2 extracted oil-tea camellia seed oil showed the highest inhibition rate on auricular swelling of ACD mice,and the inflammatory factors,such as TNF-α,IL-1β and IL-6 were reduced more than 10％compared with positive control.The degree of inflammatory infiltration significantly improved,and there was no significant difference in NF-κB positive expression of auricular tissue between supercritical CO2 extracted oil-tea camellia seed oil treatment group and negative control.However,the anti-inflammatory effect of prophylactic administration was generally lower than that of post modeling administration.The oil-tea camellia seed oil that supplemented with multiple squalene and tocopherol increased the inhibition rate of auricular swelling about 50％.There was no significant difference in NF-KB positive expression of auricular tissue between the squalene and tocopherol group and the negative control.In general,supercritical CO2 extracted oil-tea camellia seed oil exhibits the best therapeutic effect on ACD mice,and the active anti-inflammatory ingredients are closely related to squalene and tocopherol.

oil-tea camellia seed oilanti-inflammatoryallergic contact dermatitissupercritical CO2 extraction

陈宇、杨纪元、邱之阳、张笑、邱昌扬、周凯、郝泽金、陈志敏

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九江学院江西油茶研究中心/药学与生命科学学院/九江市食品加工与安全重点实验室,江西九江 332005

岭南现代农业科学与技术广东省实验室河源分中心,广东河源 517001

江西神州通油茶科技有限公司,江西 九江 332801

江西晟启农业科技有限公司,江西 九江 332621

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油茶籽油 抗炎 变应性接触性皮炎 超临界CO2萃取

岭南现代农业科学与技术广东省实验室河源分中心江西省卫生健康委科技计划项目江西省中医药管理局科技计划项目江西省教育厅科学技术研究项目江西省林业局油茶专项项目

DT20220005SKJP2202015882020A0373GJJ211804YCYJZX[2023]333

2024

10.19902/j.cnki.zgyz.1003-7969.230121

中国油脂
国家粮食储备局西安油脂科学研究设计院

中国油脂

CSTPCD北大核心
影响因子：0.842
ISSN：1003-7969
年,卷(期)：2024.49(7)