Porcess optimization of lipase Lipozyme TL IM hydrolysis of tuna oil and its lipid analysis
In order to provide a reference for the research and application of Lipozyme TL IM in the modification of fish oil and related functional products,tuna oil was used as raw material,and the immobilized lipase Lipozyme TL IM was used for hydrolysis,and the modified hydrolyzed glycerides were separated.The effects of pH,water-oil ratio(mass ratio),hydrolysis temperature and hydrolysis time on the degree of hydrolysis were studied by single factor experiments,and the appropriate hydrolysis process parameters were determined.In addition,the fatty acid composition,structure,thermal stability and oxidation stability of the modified hydrolyzed glycerides under different degrees of hydrolysis(20％-50％)were analyzed.The results showed that the suitable process parameters for hydrolysis of tuna oil by Lipozyme TL IM was pH 6.0,water-oil ratio 3∶1,hydrolysis temperature 40℃ and lipase dosage 5％(based on mass of tuna oil).Under these conditions,after hydrolysis for 30,60,80 min and 120 min,the modified hydrolyzed glycerides with hydrolysis degrees of 20％,30％,40％and 50％were obtained,respectively.With the increase of the hydrolysis degree,the polyunsaturated fatty acid content of modified hydrolyzed glycerides increased,the saturated fatty acid content decreased,the structure changed,and the thermal stability and oxidation stability deteriorated.In conclusion,Lipozyme TL IM can be used to hydrolyze tuna oil to enrich polyunsaturated fatty acids,and provide specific substrates for subsequent product development.
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