辣木籽粗多糖的分级提取及抗氧化活性
Graded extraction and antioxidant activity of crude polysaccharides from Moringa oleifera seeds
李玲玲 1徐妍然 1牛延菲 2金春秀 1胡玲华 1吴春华1
作者信息
- 1. 西南林业大学材料与化学工程学院,昆明 650224
- 2. 西南林业大学材料与化学工程学院,昆明 650224;云南省药物研究所,昆明 650111
- 折叠
摘要
为了更好地开发与利用辣木籽,以辣木籽为原料,研究了不同体积分数乙醇(20％、40％、60％、80％)分级醇沉得到的辣木籽粗多糖(P20、P40、P60、P80)的单糖组成差异以及抗氧化活性,并采用紫外可见光谱(UV-Vis)和傅里叶红外光谱(FT-IR)对纯化后的辣木籽多糖结构进行表征.结果表明:4种辣木籽粗多糖的主要成分均为鼠李糖(Rha)、半乳糖(Gal)和葡萄糖(Glc),其中粗多糖P80中单糖总含量最高(84.09 μg/mg);在4种辣木籽粗多糖中,P20(1 mg/mL)对DPPH自由基和超氧阴离子自由基清除率均最高,分别为50.24％、34.54％,P40(1 mg/mL)对羟自由基清除率最高,为76.92％,P80的总还原力最强;通过UV-Vis和FT-IR表征可知,不同体积分数乙醇能够对辣木籽粗多糖进行分级醇沉.综上,采用不同体积分数乙醇进行醇沉的辣木籽粗多糖有不同的抗氧化活性,需要根据具体应用有针对性地提取.
Abstract
In order to better develop and utilise Moringa oleifera seeds,the differences in the composition of key monosaccharides as well as antioxidant activity of crude polysaccharides(P20,P40,P60,P80)from Moringa oleifera seeds obtained by graded alcohol precipitation with different volume fraction of ethanol(20％,40％,60％,80％)were investigated,and the structure of purified polysaccharides of Moringa oleifera seeds was characterized using ultraviolet-visible spectroscopy(UV-Vis)and Fourier transform-infrared spectroscopy(FT-IR).The results showed that the main components of the four crude polysaccharides were rhamnose(Rha),galactose(Gal)and glucose(Glc),among which the crude polysaccharide P80 had the highest content of total monosaccharides(84.09 μg/mg);among the four crude polysaccharides of Moringa oleifera seeds,P20(1 mg/mL)had the highest scavenging rate of DPPH radicals and superoxide anion radicals,with 50.24％and 34.54％,respectively,P40(1 mg/mL)had the highest scavenging rate of hydroxyl radicals(76.92％),and P80 had the strongest total reducing power;it was known that different volume fraction of ethanol were able to carry out graded alcohol precipitation of crude polysaccharides from Moringa oleifera seeds by UV-Vis and FT-IR.In summary,crude polysaccharides extracted with different volume fraction of ethanol have different antioxidant activities and need to be extracted according to specific applications.
关键词
辣木籽/多糖/分级醇沉/抗氧化活性Key words
Moringa oleifera seed/polysaccharide/grading alcohol precipitation/antioxidant activity引用本文复制引用
基金项目
国家重点研发计划(2017YFC1702506)
出版年
2024
NETL
NSTL
万方数据