Metabolomics analysis of kernels of Camellia oleifera fruit treated with different methods after harvest
In order to find the optimal postharvest treatment of Camellia oleifera fruit,and lay the foundation for subsequent research,liquid chromatography-tandem mass spectrometry(LC-MS/MS)was used to determine the metabolites of the kernels of different postharvest treatments of Camellia oleifera fruit[without treatment(F8),composting treatment(FC),sun dried without shell(NS),and sun dried with shell(WS)]for 10 d and 30 d,and relevant software was used to analyze the metabolites.The results showed that a total of 1 107 characteristic metabolites were identified,with 763 and 344 metabolites identified in the positive and negative ion modes,respectively.The main metabolites were lipids and lipid-like molecules(277 kinds),phenylpropanoids and polykettides(193 kinds),organic acids and derivatives(172 kinds),benzenoids(97 kinds),organoheterocyclic compounds(94 kinds),organic oxygen compounds(84 kinds),nucleosides,nucleotides and analogues(33 kinds).By comparison of the unsaturated fatty acid of differential metabolites,six unsaturated fatty acids,namely linoleic acid,cis-9-hexadecenoic acid,8(9)-epoxy-5Z,11Z,14Z-eicosatrienoic acid,linolenic acid,ricinolic acid,9,10-epoxy-octadecenoic acid,were detected in 30 d(NS2)and 10 d(NS1)of the sun dried without shell treatment,and their expressions were up-regulated.The enrichment analysis of differential metabolites results showed that the main metabolic pathways included phenylalanine metabolism,flavonoid biosynthesis,glycerol phospholipid metabolism,glycerol ester metabolism,zeatin biosynthesis,plant secondary metabolites biosynthesis,glutathione metabolism,amino acid biosynthesis,and secondary metabolites biosynthesis.Overall,the best postharvest treatment of Camellia oleifera fruit is sun dried without shell treatment for 30 d.
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