Analysis of genetic diversity and population genetic structure in oil palm based on SSR markers
In order to provide reference for the exploration of excellent oil palm germplasm,30 pairs of SSR primers were screened to detect and analyze the genetic diversity and population genetic structure of 605 oil palm germplasm resources.The results showed that the mean value of number of observed alleles(Na)was 11.933,the mean value of effective number of alleles(Ne)was 4.116,and the mean value of polymorphism information content(PIC)was0.711 for the30 pairs of SSR primers across all resources,indicating that SSR primers were highly polymorphic and rich in genetic diversity among germplasm.The 605 germplasms were divided into two groups by Structure analysis,which was consistent with the results of principal coordinate analysis.The genetic diversity was higher in cluster 1 than that in cluster 2,and the degree of self-fertilization was significantly higher in cluster 2 than that in cluster 1.The 605 germplasms were divided into three groups by UPGMA cluster analysis,and 94%of the genetic distance between the two germplasm ranged from 0.4 to 0.8,indicating that the genetic composition of the population was simple.The molecular variance analysis showed that the intra-individual variation(85%)was higher than the inter-individual variation(10%)and inter-population(5%),which was consistent with the results of low genetic differentiation coefficient(0.025)and high gene flow(9.613)between the two groups.In general,the genetic structure of the tested germplasm population in this study is simple,the degree of genetic differentiation at the population level is low,and the genetic variation at the individual level is rich,which is urgent to pay attention to the selection and creation of mutant superior plants.
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