Objective To compare the differences in the composition of microbiota between the lung and oral cavities of lung cancer patients,and to quantify the effect of stochastic processes on the microbiota of these two sites.Methods A total of 90 lung cancer patients who were treated at Peking University Cancer Hospital&Institute between February and September 2022 were enrolled in this study.Sample DNA was extracted and sequenced after amplifying the 16S rRNA V3-V4 regions.The diversity of the oral microbiota was compared among patients with different TNM stages(Ⅰ-Ⅲ A and Ⅲ B-Ⅳ).Combined with the previous BALF sequencing data of our study,R4.1.0 software was used to compare the microbiota diversity in BALF and saliva samples,analyze the microbiota composition,and the differences in the top ten genera in relative abundance between groups.The Neutral Community Model(NCM)was applied to quantify the influence of stochastic processes on the lung and oral microbiota of lung cancer patients.Results There was no significant difference in Simpson index between group Ⅰ-Ⅲ A andⅢB-Ⅳ group(P＞0.05).The Chao1 index and β-diversity showed significant differences between the Ⅰ-Ⅲ A and Ⅲ B-Ⅳ groups(P＜0.05).Comparing the characteristics of lung and oral microbial microbiota in lung cancer patients,there were significant differences in αand β diversity between the two types samples from different sources(P＜0.05).The NCM estimated a partial relationship between the frequency of ASVs and changes of relative abundance,explaining 5.7％,61.7％,and 41.5％of the microbial community variation in BALF,saliva,and both samples,respectively.Conclusion The results of the NCM suggest that stochastic processes have a greater impact on oral microbiota,indicating that the migration rate of oral microbiota is higher than that of lung microbiota in lung cancer patients.
Lung neoplasmOral microbiota16S rRNANeutral community model
维普
万方数据
