首页|嗜热厌氧菌共培养发酵玉米秸秆产氢的研究

嗜热厌氧菌共培养发酵玉米秸秆产氢的研究

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氢能是对环境无害且可以替代化石燃料的可持续性能源.利用嗜热厌氧菌暗发酵木质纤维素生产氢气是一种极具潜力的生物制氢技术,具有清洁、高效和可再生的优势.构建解糖热解纤维素菌和热解糖厌氧杆菌共培养体系,考察两株菌株接种比例、总接种量和底物浓度对玉米秸秆发酵产氢的影响.实验结果表明,在发酵体系初始pH值7.0,培养温度60 ℃条件下,当解糖热解纤维素菌和热解糖厌氧杆菌接种比例为3∶2,菌种总接种量为6%,秸秆浓度为15 g·L-1时,体系产氢能力最强.此时,发酵体系产氢量累积达到65.6 mL·g-1-秸秆,氢气含量为46.9%,最大产氢速率为1.47 mL·g-1h-1.
A Study on Hydroger Production from Corn Stran by Co-culture of Thermophilic Anaerobic Bacteria
Hydrogen energy is a sustainable energy source that is environmentally friendly and can replace fossil fuels.The use of thermophilic anaerobic bacteria for dark fermentation of lignocellulose to produce hydrogen is a highly promising bio-logical hydrogen production technology,with the advantages of cleanliness,efficiency,and renewability.This study con-structed a co-culture system of Caldicellulosiruptor saccharolyticus and Thermoanaerobacterium thermosaccharolyticum,and then investigated effects of inoculation ratio of the two strains,total inoculation amount,and substrate concentration on hy-drogen production during corn straw fermentation.The experimental results showed that under the conditions of initial pH 7.0 and cultivation temperature of 60 ℃,hydrogen production capacity of fermentation system was strongest when inocula-tion ratio of the two strains was 3:2,total inoculation amount was 6%,and straw concentration was 15 g·L-1.Under this condition,the hydrogen production reached 65.6 mL·g-1-straw,and the hydrogen content was 46.9%,the maximum hy-drogen production rate achieved 1.47 mL·g-1 h-1.

thermophilic anaerobic bacteriacorn strawfermentative hydrogen productionhydrogen production evaluation

黎霞、邹淑琦、徐延亮、贺静

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农业农村部成都沼气科学研究所,农业农村部农村可再生能源开发利用重点实验室,四川成都 610041

嗜热厌氧菌 秸秆 发酵产氢 产氢分析

四川省科技厅重点研发项目四川省区域创新合作项目国家现代农业产业技术体系四川创新团队项目

2022YFS04792023YFQ0030ccxtd-2020-7

2024

中国沼气
中国沼气学会 农业部沼气科学研究所

中国沼气

CSTPCD
影响因子:0.738
ISSN:1000-1166
年,卷(期):2024.42(1)
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