Hydrogen energy is a sustainable energy source that is environmentally friendly and can replace fossil fuels.The use of thermophilic anaerobic bacteria for dark fermentation of lignocellulose to produce hydrogen is a highly promising bio-logical hydrogen production technology,with the advantages of cleanliness,efficiency,and renewability.This study con-structed a co-culture system of Caldicellulosiruptor saccharolyticus and Thermoanaerobacterium thermosaccharolyticum,and then investigated effects of inoculation ratio of the two strains,total inoculation amount,and substrate concentration on hy-drogen production during corn straw fermentation.The experimental results showed that under the conditions of initial pH 7.0 and cultivation temperature of 60 ℃,hydrogen production capacity of fermentation system was strongest when inocula-tion ratio of the two strains was 3:2,total inoculation amount was 6％,and straw concentration was 15 g·L-1.Under this condition,the hydrogen production reached 65.6 mL·g-1-straw,and the hydrogen content was 46.9％,the maximum hy-drogen production rate achieved 1.47 mL·g-1 h-1.
关键词
嗜热厌氧菌/秸秆/发酵产氢/产氢分析
Key words
thermophilic anaerobic bacteria/corn straw/fermentative hydrogen production/hydrogen production evaluation
维普
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