Isolation and Identification Bacteria for Efficient Removal of Amine Nitrogen from Fermentation Mattress Materi-al
Ammonia emissions is one of the most common and harmful odors produced by poultry manure in chicken farms,causing losses to production.Using bacteria with function of degrading ammonia nitrogen can inhibit the ammonia emis-sions.In this study,4 bacterial strains were isolated from chicken-raising fermentation material using culture medium with(NH4)2SO4 as the sole nitrogen source.Based on phylogenetic analysis of 16SrDNA sequences,strains FJAT-56390 and FJAT-56388 belong to the genus Cellulosimicrobium,and theyare grouped together with C.aquatile strain 3bp(NR146008)at a same basal clade of the phylogenetic tree.The strain FJAT-56389 isthemost similar to Bacillus cereus ATCC 1459(NR074540),with FJAT-56387 to Bacillus amyloliquefaciens(MK182997)in the phylogenetictree.The con-centration changes of ammonia nitrogen of mediawere measuredby the method of salicylic acid spectrophotometry.The re-moval rate of ammonia nitrogen was 84％ by FJAT-54390in 96 hours,nonsignificantly higher than 79％of removal rateby FJAT-56387(p=0.1631),and significantly higher than that by the other two strains with removal rates of57％and 79％.This study indicates that FJAT-54390 is a promising microbial agentto mitigate ammonia emissions and it is worthy of further research.
fermentation mattressremoval of ammoniaCellulosimicrobiumBacillus
万方数据
维普
