产甲烷古菌甲基转移酶研究进展
Advances in Methyltransferases from Methanogens
杨清 1白丽萍1
作者信息
- 1. 农业农村部成都沼气科学研究所,成都 610041;农业农村部可再生能源开发利用重点实验室,成都 610041
- 折叠
摘要
产甲烷古菌是一类能够通过产甲烷代谢生长的严格厌氧微生物.甲基营养型产甲烷古菌主要通过利用甲基类化合物如甲醇、甲胺、甲硫醚、甲氧基芳香族化合物等产CH4.除了 H2依赖性甲基还原途径外,其余所有产甲烷途径中均含有MtrA-H甲基转移酶,而甲基营养型产甲烷途径还包含底物特异性的甲基转移酶,负责裂解底物产生甲基并将其转移到一碳载体HS-CoM或H4MPT.这些甲基转移酶在识别底物的蛋白结构上存在差异,但它们都含有高度保守的类咕啉结合蛋白以及辅因子cobalamin.总结了产甲烷古菌中甲基转移酶的分类、生理功能、结构特征及催化机制的研究进展,并对未来甲基转移酶的研究方向进行了展望.
Abstract
Methanogenic archaea are strictly anaerobic organisms that grow by producing methane.Methylotrophic methano-genic archaea predominantly generate CH4 by metabolizing methyl compounds such as methanol,methylamine,methyl sul-fide,and methylated aromatic substances.Except for the hydrogen-dependent methylotrophic pathway,MtrA-H methyl-transferases exist in all methane-producing pathways,methylotrophic pathways moreover contain substrate-specific methyl-transferases that are responsible for both cleaving and transferring methyl groups to HS-CoM or H4MPT.Despite significant adjustments to the protein architectures involved in substrate recognition between methyltransferases,they all contain highly conserved corrinoid-binding proteins and the cofactor cobalamin.This article outlines the classification,biological func-tions,structural properties,and catalytic mechanisms of methyltransferases in methanogenic archaea,and also discusses fu-ture directions in methyltransferase research.
关键词
产甲烷古菌/甲基转移酶/蛋白结构/催化机制Key words
methanogens/methyltransferases/protein Structure/catalytic Mechanism引用本文复制引用
基金项目
国家自然科学基金(31970066)
中央级公益性科研院所基本科研业务费专项(Y2021XK06)
四川省国际科技创新合作项目(2021YFH0049)
中国农科院基本科研业务费装修项目(1610032019001-03101)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据