High-temperature Intensified High-concentration Anaerobic Fermentation of Cow Manure Synergizes with the Re-duction of Antibiotic Resistance Genes
In order to improve the efficiency of anaerobic digestion(AD)of livestock and poultry manure and promote its stable operation under high organic load conditions,the growth and decline mechanism of antibiotic resistance genes(ARGs)during the anaerobic fermentation process was explored through sequencing;A batch experiment was carried out to study the effects of the gas production performance,functional microbial communities and ARGs of high-concentration anae-robic fermentation of cow manure(12％TS)at two process temperatures of medium temperature 35 ℃ and high tempera-ture 55 ℃,and explore the relationship between different factors and ARGs;The cow dung high solid concentration fer-mentation system has better gas production performance at high temperature of 55 ℃,and the methane production increases by 17.27％.The main components of VFAs during medium-temperature fermentation are acetic acid and propionic acid,which account for approximately 55.58％of VFAs.Among them,the accumulation of propionic acid is high,the conver-sion is slow,and the system is temporarily acidified.The high-temperature fermentation system has stronger stability and acid resistance.High temperature changes the dominant bacterial genera during the fermentation process,increases the a-bundance of Acetivibrio,Petrimonas,Methanothermobacter and other microorganisms,thereby increasing gas production and maintaining the dynamic balance of acidogenic bacteria and methanogenic bacteria.Compared with medium temperature(35 ℃),high temperature(55 ℃)fermentation can promote the reduction of the relative abundance of ARGs(especially aph(6)-Id,aph(3")-Ib and sul1);Increasing the temperature can significantly improve the gas production performance of the anaerobic fermentation system and increase stability;Changes in the microbial community are the main driving factors for the growth and decline of ARGs.The research results can provide reference for process optimization of anaerobic fermenta-tion of livestock and poultry breeding manure.
high temperaturecow manureanaerobic digestion systems with high substrate concentrationsanaerobic mi-crofloraantibiotic resistance genes
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