首页|Effects of Panax Quinquefolium Saponin on Phosphatidylinositol 3-Kinase/Serine Threonine Kinase Pathway of Neonatal Rat Myocardial Cells Subjected to Hypoxia

Effects of Panax Quinquefolium Saponin on Phosphatidylinositol 3-Kinase/Serine Threonine Kinase Pathway of Neonatal Rat Myocardial Cells Subjected to Hypoxia

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Objective:To explore the effects of Panax Quinquefolium Saponin (PQS) on phosphatidylinositol 3-kinase/serine threonine kinase (PI3K/Akt) pathway of neonatal rat myocardial cells subjected to hypoxia.Methods:Neonatal rat myocardial cells were cultured in vitro.After the myocardial cell injury was induced by hypoxia,the cells were randomized into 5 groups:the normal group,the model group,the positive control group (Ciclosporin A,2 μ mol/L),the low-dose PQS group (PQSL,25mg/L),and the high-dose PQS group (PQSH,50 mg/L).Morphology and behavior of myocardial cells were observed under an inverted microscope.Apoptosis rate and lactate dehydrogenase (LDH) leakage rate of myocardial cells were determined by colorimetry.Mitochondrial transmembrane potential was assessed using a fluorexon laser.Phospho-glycogen synthase kinase (GSK)-3 β and phospho-Akt as well as cytochrome C were determined by Westem blot.Results:LDH leakage in the Ciclosporin A group,PQSH group and PQSL group reduced progressively compared with the model group (P＜0.05).Akt and GSK-3 β was strongly phosphorylated after treatment with Ciclosporin A and PQS compared with the model group (P＜0.05,P＜0.01).Compared with the model group (16.41 ± 1.74;35.28±6.30),both the integrated optical density of mitochondrial permeability transition pore (MPTP) and the mitochondrial transmembrane potential significantly increased in the PQSH group (42.74±2.12;71.36±6.54) and the PQSL group (39.58 ± 1.49;66.99 ± 5.45;P＜0.05,P＜0.01).However,the protein of cytochrome C outside the mitochondrion decreased in the PQSH group (273.66± 14.61) and the PQSL group (259.62 ± 17.31) compared with the model group (502.41 ± 17.76;P＜0.05).Conclusion:Through activation of the PI3K/Akt pathway and inhibition of the MPTP,PQS might protect the heart against ischemia injury and apoptosis of myocardial cells.

Panax Quinquefolium Saponinischemic cardiomyocytesphosphatidylinositol 3-kinase/serine threonine kinasemitochondrial permeability transition pore

GUO Chun-yu、MA Xiao-juan、WANG Jing-shang、SHI Ying、LIU Xin、YIN Hui-jun、CHEN Ke-ji

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Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing 100091, China

China Postdoctoral Science FondationNational Natural Science Fondation of China

2010048042781041038

2015

10.1007/s11655-014-1881-8

中国结合医学杂志(英文版)
中国中西医结合学会 中国中医研究院

中国结合医学杂志(英文版)

CSCDSCI
影响因子：1.056
ISSN：1672-0415
年,卷(期)：2015.21(5)
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