首页|Inflammatory and Immunomodulatory Effects of Tripterygium wilfordii Multiglycoside in Mouse Models of Psoriasis Keratinocytes
Inflammatory and Immunomodulatory Effects of Tripterygium wilfordii Multiglycoside in Mouse Models of Psoriasis Keratinocytes
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Objective:To determine the role of Tripterygium wilfordii multiglycoside(TGW)in the treatment of psoriatic dermatitis from a cellular immunological perspective.Methods:Mouse models of psoriatic dermatitis were established by imiquimod(IMQ).Twelve male BALB/c mice were assigned to IMQ or IMQ+TGW groups according to a random number table.Histopathological changes in vivo were assessed by hematoxylin and eosin staining.Ratios of immune cells and cytokines in mice,as well as PAM212 cell proliferation in vitro were assessed by flow cytometry.Pro-inflammatory cytokine expression was determined using reverse transcription quantitative polymerase chain reaction.Results:TGW significantly ameliorated the severity of IMQ-induced psoriasis-like mouse skin lesions and restrained the activation of CD45+cells,neutrophils and T lymphocytes(all P＜0.01).Moreover,TGW significantly attenuated keratinocytes(KCs)proliferation and downregulated the mRNA levels of inflammatory cytokines including interleukin(IL)-17A,IL-23,tumor necrosis factor α,and chemokine(C-X-C motif)ligand 1(P＜0.01 or P＜0.05).Furthermore,it reduced the number of γ δT17 cells in skin lesion of mice and draining lymph nodes(P＜0.01).Conclusions:TGW improved psoriasis-like inflammation by inhibiting KCs proliferation,as well as the associated immune cells and cytokine expression.It inhibited IL-17 secretion from γδ T cells,which improved the immune-inflammatory microenvironment of psoriasis.
psoriasisTripterygium wilfordii multiglycosideγ δ T cellsinflammationChinese medicine
Department of Dermatology,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai(200437),China
Institute of Dermatology,Shanghai Academy of Traditional Chinese Medicine,Shanghai(201203),China
Department of Integrated Traditional Chinese and Western Medicine,Shanghai Skin Disease Hospital,Tongji University School of Medicine,Shanghai(200443),China
National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaShanghai Pujiang Talent ProgramScience and Technology Commission of Shanghai MunicipalityScience and Technology Commission of Shanghai Municipality
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