首页|Protective Effects of Danmu Extract Syrup on Acute Lung Injury Induced by Lipopolysaccharide in Mice through Endothelial Barrier Repair

Protective Effects of Danmu Extract Syrup on Acute Lung Injury Induced by Lipopolysaccharide in Mice through Endothelial Barrier Repair

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Objective:To investigate the effects of Danmu Extract Syrup(DMS)on lipopolysaccharide(LPS)-induced acute lung injury(ALI)in mice and explore the mechanism.Methods:Seventy-two male Balb/C mice were randomly divided into 6 groups according to a random number table(n=12),including control(normal saline),LPS(5 mg/kg),LPS+DMS 2.5 mL/kg,LPS+DMS 5 mL/kg,LPS+DMS 10 mL/kg,and LPS+Dexamethasone(DXM,5 mg/kg)groups.After pretreatment with DMS and DXM,the ALI mice model was induced by LPS,and the bronchoalveolar lavage fluid(BALF)were collected to determine protein concentration,cell counts and inflammatory cytokines.The lung tissues of mice were stained with hematoxylin-eosin,and the wet/dry weight ratio(W/D)of lung tissue was calculated.The levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-1βin BALF of mice were detected by enzyme linked immunosorbent assay.The expression levels of Claudin-5,vascular endothelial(VE)-cadherin,vascular endothelial growth factor(VEGF),phospho-protein kinase B(p-Akt)and Akt were detected by Western blot analysis.Results:DMS pre-treatment significantly ameliorated lung histopathological changes.Compared with the LPS group,the W/D ratio and protein contents in BALF were obviously reduced after DMS pretreatment(P＜0.05 or P＜0.01).The number of cells in BALF and myeloperoxidase(MPO)activity decreased significantly after DMS pretreatment(P＜0.05 or P＜0.01).DMS pre-treatment decreased the levels of TNF-α,IL-6 and IL-1β(P＜0.01).Meanwhile,DMS activated the phosphoinositide 3-kinase/protein kinase B(PI3K/Akt)pathway and reversed the expressions of Claudin-5,VE-cadherin and VEGF(P＜0.01).Conclusions:DMS attenuated LPS-induced ALI in mice through repairing endothelial barrier.It might be a potential therapeutic drug for LPS-induced lung injury.

Danmu Extract Syrupacute lung injuryinflammatory factorsvascular endothelial growth factorphosphoinositide 3-kinase/protein kinase BChinese medicineNauclea officinalis

XU Han、XU Si-cong、LI Li-yan、WU Yu-huang、TAN Yin-feng、CHEN Long、LIU Pei、LIANG Chang-fu、HE Xiao-ning、LI Yong-hui

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Hainan Provincial Key Lab of Research & Development on Tropic Herbs,Hainan Medical University,Haikou(571199),China

Department of Stomatology,the Second Affiliated Hospital of Hainan Medical University,Haikou(571199),China

National Natural Science Foundation of ChinaHainan Provincial Natural Science Foundation of China

821608262019RC207

2024

10.1007/s11655-023-3604-5

中国结合医学杂志(英文版)
中国中西医结合学会 中国中医研究院

中国结合医学杂志(英文版)

CSTPCD
影响因子：1.056
ISSN：1672-0415
年,卷(期)：2024.30(3)
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