首页|Paeoniflorin,the Main Monomer Component of Paeonia lactiflora,Exhibits Anti-inflammatory Properties in Osteoarthritis Synovial Inflammation

Paeoniflorin,the Main Monomer Component of Paeonia lactiflora,Exhibits Anti-inflammatory Properties in Osteoarthritis Synovial Inflammation

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Objective:To explore the mechanism of paeoniflorin(PF)on osteoarthritis(OA)synovial inflammation from network pharmacology to experimental pharmacology.Methods:Targets of OA were constructed by detecting the database of network database platforms(Therapeutic Target database,DrugBank and GeneCards),and the targets of PF were constructed by PubChem and Herbal Ingredients'Targets database.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis of these co-targeted genes were conducted via Database for Annotation,Visualization,and Integrated Discovery(DAVID)database,and protein-protein interaction(PPI)networks were conducted via the search tool for the retrieval of interacting genes(STRING)database.Cell counting kit-8(CCK-8)assay was performed to assess the potential toxicity of PF on human OA fibroblast-like synoviocytes(FLS),quantitative real-time polymerase chain reaction(qPCR),enzyme-linked immunosorbent assay(ELISA)and Western blot were used to verify the potential mechanism of PF in synovial inflammation.Results:Twenty-six co-targeted genes were identified.GO enrichment results showed that these co-targeted genes were most likely localized in the cytoplasm,and the biological processes mainly involved'cellular response to hypoxia1'lipopolysaccharide(LPS)-mediated signaling pathway'and'positive regulation of gene expression'.KEGG pathway analysis indicated that these co-targeted genes may function through pathways associated with'hypoxia-inducible factor-1(HIF-1)signaling pathway'and'tumor-necrosis factor(TNF)signaling pathway'.The PPI network showed that the top 3 hub genes were TP53,TNF,and CASP3.Molecular docking results showed that PF was well docking with TNF.CCK-8 showed no potential toxicity of 10,20 and 50 pmol/L PF on human OA FLS.And PF significantly decreased the expression levels of interleukin-1 β,interleukin-6,TNF-α matrix metalloproteinase 13(MMP13),and a disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5)and TNF-α in LPS-induced OA FLS.Conclusion:PF exhibited potent anti-inflammatory effect in OA synovial inflammation.

paeoniflorinosteoarthritissynovial inflammationtumor necrosis factor-αnetwork pharmacologyPaeonia lactiflora

CHEN Pu、ZHOU Jun、RUAN An-min、MA Yu-feng、WANG Qing-fu

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Department of Orthopaedic Surgery,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing(100029),China

Department of Orthopaedic Surgery,Guangdong Provincial Hospital of Chinese Medicine,Guangzhou(510120),China

Department of Orthopaedic Surgery,Beijing Longfu Hospital,Beijing(100010),China

国家自然科学基金国家自然科学基金Capacity Building Project of Chinese and Western Medicine Clinical Collaboration on Major Difficult Disease

8137366281874475201803190106

2024

10.1007/s11655-023-3653-9

中国结合医学杂志(英文版)
中国中西医结合学会 中国中医研究院

中国结合医学杂志(英文版)

CSTPCD
影响因子：1.056
ISSN：1672-0415
年,卷(期)：2024.30(5)
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