首页|Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway

Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway

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Objective:To explore the regulatory effect of Pien Tze Huang(PZH)on targeting partner of NOB1(PNO1)and it's down-stream mediators in colorectal cancer(CRC)cells.Methods:Quantitative polymerase chain reaction was performed to determine mRNA levels of PNO1,TP53,and CDKN1A.Western blotting was performed to determine protein levels of PNO1,p53,and p21.HCT-8 cells were transduced with a lentivirus over-expressing PNO1.Colony formation assay was used to detect cell survival in PNO1 overexpression of HCT-8 cells after PZH treatment.Cell-cycle distribution,cell viability and cell apoptosis were performed to identify the effect of PNO1 overexpression on cell proliferation and apoptosis of HCT-8 cells after PZH treatment.Xenograft BALB/c nude mice bearing HCT116 cells transduced with sh-PNO1 or sh-Ctrl lentivirus were evaluated.Western blot assay was performed to detect PNO1,p53,p21 and PCNA expression in tumor sections.Terminal deoxynucleotidyl transferase dUTP nick end labling(TUNEL)assay was used to determine the apoptotic cells in tissues.Results:PZH treatment decreased cell viability,down-regulated PNO1 expression,and up-regulated p53 and p21 expressions in HCT-8 cells(P＜0.05).PNO1 overexpression attenuated the effects of PZH treatment,including the expression of p53 and p21,cell growth,cell viability,cell cycle arrest and cell apoptosis in vitro(P＜0.05).PNO1 knockdown eliminated the effects of PZH treatment on tumor growth,inhibiting cell proliferation inhibition and apoptosis induction in vivo(P＜0.05).Similarly,PNO1 knockdown attenuated the effects of PZH treatment on the downregulation of PNO1 and up regulation of p53 and p21 in vivo(P＜0.05).Conclusion:The mechanism by which PZH induces its CRC anti-proliferative effect is at least in part by regulating the expression of PNO1 and its downstream targets p53 and p21.

colorectal cancerPien Tze HuangChinese medicinepartner of NOB1proliferationp53/p21 pathway

CAO Liu-jing、LIU Li-ya、CHEN You-qin、HAN Yu-ying、WEI Li-hui、YAO Meng-ying、FANG Yi、WU Mei-zhu、CHENG Ying、Thomas J.Sferra、LIU Hui-xin、LI Li、PENG Jun、SHEN A-ling

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Clinical Research Institute,the Second Affiliated Hospital &Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou(350122),China

Fujian Key Laboratory of Integrative Medicine in Geriatrics,Fujian University of Traditional Chinese Medicine,Fuzhou(350122),China

Department of Pediatrics,Case Western Reserve University School of Medicine,UH Rainbow Babies and Children's Hospital,Cleveland,OH(44106),USA

Innovation and Transformation Center,Fujian University of Traditional Chinese Medicine,Fuzhou(350122),China

Department of Health Management,Fujian Provincial Hospital,Shengli Clinical College of Fujian Medical University,Fuzhou(350001),China

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国家自然科学基金国家自然科学基金国家自然科学基金福建省自然科学基金

8180388282274188822741482020J06026

2024

10.1007/s11655-024-3709-5

中国结合医学杂志(英文版)
中国中西医结合学会 中国中医研究院

中国结合医学杂志(英文版)

CSTPCD
影响因子：1.056
ISSN：1672-0415
年,卷(期)：2024.30(6)
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