UPLC-Q-TOF/MS-Based Serum Metabolomics Reveals Potential Anti-tumor Mechanism of Banxia Xiexin Decoction in Colorectal Cancer Mice

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外文摘要：Objective:To clarify the potential mechanism of Banxia Xiexin Decoction(BXD)on colorectal cancer(CRC)from the perspective of metabolomics.Methods:Forty male C57BU6 mice were randomly divided into normal control(NC),azoxymethane/dextran sulfate sodium(AOM/DSS)model,low-dose BXD(L-BXD),high-dose BXD(H-BXD)and mesalamine(MS)groups according to a random number table,8 mice in each group.Colorectal cancer model was induced by AOM/DSS.BXD was administered daily at doses of 3.915(L-BXD)and 15.66 g/kg(H-BXD)by gavage for consecutive 21 days,and 100 mg/kg MS was used as positive control.Following the entire modeling cycle,colon length of mice was measured and quantity of colorectal tumors were counted.The spleen and thymus index were determined by calculating the spleen/thymus weight to body weight.Inflammatory cytokine and changes of serum metabolites were analyzed by enzyme-linked immunosorbent assay kits and ultra performance liquid chromatography-quadrupole/time-of-flight mass spectrometry(UPLC-Q/TOF-MS),respectively.Results:Notably,BXD supplementation protected against weight loss,mitigated tumor formation,and diminished histologic damage in mice treated with AOM/DSS(P＜0.05 or P＜0.01).Moreover,BXD suppressed expression of serum inflammatory enzymes,and improved the spleen and thymus index(P＜0.05).Compared with the normal group,102 kinds of differential metabolites were screened in the AOM/DSS group,including 48 potential biomarkers,involving 18 main metabolic pathways.Totally 18 potential biomarkers related to CRC were identified,and the anti-CRC mechanism of BXD was closely related to D-glutamine and D-glutamate metabolism,phenylalanine,tyrosine and tryptophan biosynthesis,arginine biosynthesis,nitrogen metabolism and so on.Conclusion:BXD exerts partial protective effects on AOM/DSS-induced CRC by reducing inflammation,protecting organism immunity ability,and regulating amino acid metabolism.

外文关键词：

colorectal cancermetabolomicsultra performance liquid chromatography-quadrupole/time-of-flight mass spectrometryazoxymethanedextran sulfate sodiumBanxia Xiexin DecoctionChinese medicine

作者：

YUE Yin-zi、LI Ming-xuan、WANG Xiao-hui、QIN Yuan-yuan、WANG Ya-hui、TAN Jin-hua、SU Lian-lin、YAN Shuai

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作者单位：

Department of General Surgery,Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Suzhou(215009),China

School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing(210023),China

Department of General Surgery,Bayinguoleng Mongolian Autonomous Prefecture People's Hospital,Xinjiang Uygur Autonomous Region,Korla(841000),China

Department of Pharmacy,Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Suzhou(215009),China

Department of Anorectal Surgery,Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Suzhou(215009),China

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基金：

Natural Science Foundation of Nanjing University of Chinese MedicineScience and Technology Innovation Project of Suzhou Medical and Health CareFifth Batch of Gusu Health Personnel Training Project in Suzhou

项目编号：

XZR2020038SKJY2021136GSWS2020085

出版年：

2024

DOI：

10.1007/s11655-023-3552-0

中国结合医学杂志(英文版)

中国中西医结合学会中国中医研究院

中国结合医学杂志(英文版)

CSTPCD

影响因子：1.056

ISSN：1672-0415

年,卷(期)：2024.30(7)