首页|Neuroprotective Effect and Mechanism of Tanreqing Injection on Ischemic Stroke:Insights from Network Pharmacology and in vivo Experiments

Neuroprotective Effect and Mechanism of Tanreqing Injection on Ischemic Stroke:Insights from Network Pharmacology and in vivo Experiments

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Objective:To explore the neuroprotective effects and mechanism of Tanreqing Injection(TRQ)on treating ischemic stroke based on network pharmacology and in vivo experimental validation.Methods:The chemical compounds of TRQ were retrieved based on published data,with targets retrieved from PubChem,Therapeutic Target Database and DrugBank.Network visualization and analysis were performed using Cytoscape,with protein-protein interaction networks derived from the STRING database.Enrichment analysis was performed using Kyoto Encyclopedia of Genes Genomes pathway and Gene Ontology analysis.In in vivo experiments,the middle cerebral artery occlusion(MCAO)model was used.Infarct volume was determined by 2,3,5-triphenyltetrazolium hydrochloride staining and protein expressions were analyzed by Western blot.Molecular docking was performed to predict ligand-receptor interactions.Results:We screened 81 chemical compounds in TRQ and retrieved their therapeutic targets.Of the targets,116 were therapeutic targets for stroke.The enrichment analysis showed that the apelin signaling pathway was a key pathway for ischemic stroke.Furthermore,in in vivo experiment we found that administering with intraperitoneal injection of 2.5 mL/kg TRQ every 6 h could significantly reduce the infarct volume of MCAO rats(P＜0.05).In addition,protein levels of the apelin receptor(APJ)/phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)pathway were increased by TRQ(P＜0.05).In addition,41 chemical compounds in TRQ could bind to APJ.Conclusions:The neuroprotective effect of TRQ may be related to the APJ/PI3K/AKT signaling pathway.However,further studies are needed to confirm the findings.

Tanreqing InjectionChinese medicineischemic strokemiddle cerebral artery occlusionquantitative estimate of drug-likeness

LI Zhong-hao、PU Xiao-qi、LI Sha-sha、DONG Xiao-ke、ZHANG Guo-qiang、WANG Yu、LIU Jin-min

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Department of Emergency,China-Japan Friendship Hospital,Beijing(100029),China

Department of Neurology,Beijing University of Chinese Medicine Dongfang Hospital,Beijing(100078),China

Department of Radiology,China-Japan Friendship Hospital,Beijing 100029,China

Graduate School,Beijing University of Chinese Medicine,Beijing 100029,China

Department of Neurology,Beijing Daxing District Hospital of Integrated Chinese and Western Medicine,Beijing(100163),China

Department of Emergency,China-Japan Friendship Hospital,Beijing 100029,China

Department of Neurology,China-Japan Friendship Hospital,Beijing 100029,China

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2024

10.1007/s11655-024-3910-6

中国结合医学杂志(英文版)
中国中西医结合学会 中国中医研究院

中国结合医学杂志(英文版)

CSTPCD
影响因子：1.056
ISSN：1672-0415
年,卷(期)：2024.30(8)