Therapeutic Potential of Thunbergia laurifolia L.Extract in Gestational Diabetes Mellitus:Insights from a Rat Model

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外文摘要：Objective:To assess the effects of Thunbergia laurifolia L.extract(TLE)on gestational diabetes mellitus(GDM)in a rat model.Methods:Thunbergia laurifolin L.leaves were subjected to ethanolic extraction.In vivo study,50 pregnant rats were randomly divided into 5 groups(10 for each):non-GDM group,GDM induced by streptozotocin(STZ,60 mg/kg i.p.),metformin(MET)100 mg/kg,TLE 50,and 500 mg/kg groups.Administration was performed on gestation day 7 until term(day 21).The effects of TLE on blood glucose,insulin levels,lipid profiles,liver enzymes,and maternal performances were assessed.In in vitro study,the effect of TLE was examined using the organ bath for uterine force measurement.Results:In in vivo study,TLE significantly reduced blood glucose as compared to GDM(P＜0.05)with gradually increased insulin level.This effect was consistent with islets of Langerhans restoration.Histologically,the uterine muscular layer displayed a marked increase in fiber area in response to both doses as compared to GDM(P＜0.05).Additionally,TLE significantly reduced total cholesterol,triglyceride,and alanine transaminase levels(P＜0.05).Intriguingly,TLE also led to a notable augmentation in gravid uterus size,live fetuses count,and implantation numbers,while significantly reducing the post-implantation loss rate associated with fetal classification(P＜0.05).Thus,GDM improvements were dose to those produced by MET.In in vitro study,TLE exerted a concentration-dependent inhibition of spontaneous uterine contractility(half-maximal inhibition concentration=1.2 mg/L).This inhibitory effect extended to potassium chloride depolarization and oxytocin-mediated contractions.When combined with its major constituent,rosmarinic acid,TLE produced an enhanced inhibitory effect(P＜0.05).Conclusions:TLE ameliorated blood glucose levels,enhanced uterine muscular structure,and improved maternal and fetal performance in GDM.TLE also displayed tocolytic properties.These findings underscore the need for further exploration of TLE as a potential tocolytic agent to mitigate GDM-associated complications.

外文关键词：

myometriumgestational diabetes mellitustocolyticoxytocinThunbergia laurifolia L

作者：

Sasitorn Kerdsuknirund、Arreeya Kosinan、Panida Khunkaewla、Pakanit Kupittayanant、Ratchadaporn Oonsivilai、Pattama Tongdee、Porntip Nimkuntod、Susan Wray、Sajeera Kupittayanant

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作者单位：

School of Preclinical Sciences,Institute of Science,Suranaree University of Technology,Nakhon Ratchasima(30000),Thailand

Biochemistry-Electrochemistry Research Unit,School of Biochemistry,Institute of Science,Suranaree University of Technology,Nakhon Ratchasima(30000),Thailand

School of Animal Technology and Innovation,Institute of Agricultural Technology,Suranaree University of Technology,Nakhon Ratchasima(30000),Thailand

School of Food Technology,Institute of Agricultural Technology,Suranaree University of Technology,Nakhon Ratchasima 30000,Thailand

School of Obstetrics and Gynecology,Institute of Medicine,Suranaree University of Technology,Nakhon Ratchasima(30000),Thailand

School of Internal Medicine,Institute of Medicine,Suranaree University of Technology,Nakhon Ratchasima(30000),Thailand

Harris-Wellbeing Preterm Birth Research Centre,Department of Women and Children's Health,the Institute of Lifecourse and Medical Sciences,University of Liverpool,Liverpool,United Kingdom

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基金：

Institute of Research and Development,Suranaree University of Technology

项目编号：

SUT-PhD/14/2554

出版年：

2024

DOI：

10.1007/s11655-024-3764-y

中国结合医学杂志(英文版)

中国中西医结合学会中国中医研究院

中国结合医学杂志(英文版)

CSTPCD

影响因子：1.056

ISSN：1672-0415

年,卷(期)：2024.30(9)