Shexiang Tongxin Dropping Pill Promotes Angiogenesis through VEGF/eNOS Signaling Pathway on Diabetic Coronary Microcirculation Dysfunction

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外文摘要：Objective:To study the effect of Shexiang Tongxin Dropping Pill(STDP)on angiogenesis in diabetic cardiomyopathy mice with coronary microcirculation dysfunction(CMD).Methods:According to a random number table,6 of 36 SPF male C57BU6 mice were randomly selected as the control group,and the remaining 30 mice were injected with streptozotocin intraperitoneally to replicate the type 1 diabetes model.Mice successfully copied the diabetes model were randomly divided into the model group,STDP low-dose group[15 mg/(kg·d)],medium-dose group[30 mg/(kg·d)],high-dose group[60 mg/(kg·d)],and nicorandil group[15 mg/(kg·d)],6 in each group.The drug was given by continuous gavage for 12 weeks.The cardiac function of mice in each group was detected at the end of the experiment,and coronary flow reserve(CFR)was detected by chest Doppler technique.Pathological changes of myocardium were observed by hematoxylin-eosin staining,collagen fiber deposition was detected by masson staining,the number of myocardial capillaries was detected by platelet endothelial cell adhesion molecule-1 staining,and the degree of myocardial hypertrophy was detected by wheat germ agglutinin staining.The expression of the vascular endothlial growth factor(VEGF)/endothelial nitric oxide synthase(eNOS)signaling pathway-related proteins in myocardial tissue was detected by Western blot.Results:Compared with the model group,medium-and high-dose STDP significantly increased the left ventricular ejection fraction and left ventricular fraction shortening(P＜0.01),obviously repaired the disordered cardiac muscle structure,reduced myocardial fibrosis,reduced myocardial cell area,increased capillary density,and increased CFR level(all P＜0.01).Western blot showed that high-dose STDP could significantly increase the expression of VEGF and promote the phosphorylation of vascular endothelial growth factor receptor 2,phosphoinositide 3-kinase,protein kinase B,and eNOS(P＜0.05 or P＜0.01).Conclusion:STDP has a definite therapeutic effect on diabetic CMD,and its mechanism may be related to promoting angiogenesis through the VEGF/eNOS signaling pathway.

外文关键词：

Shexiang Tongxin Dropping Pilltype 1 diabetesdiabetic cardiomyopathycoronary microcirculation dysfunctionangiogenesis

作者：

CUI Xin-yu、LIU Tian-hua、BAI Ya-li、ZHANG Meng-di、LI Guo-dong、ZHANG Yu-ting、YUAN Yue-ying、ZHANG Ya-wen、YU Li-shuang、HAN Li-na、WU Yan

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作者单位：

School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing(100029),China

Key Laboratory of Traditional Chinese Medicine Syndrome and Formula Ministry of Education,Beijing University of Chinese Medicine,Beijing(100029),China

School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China

Beijing Research Institute of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China

Beijing Research Institute of Chinese Medicine,Beijing University of Chinese Medicine,Beijing(100029),China

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基金：

National Natural Science Foundation of China

项目编号：

81930113

出版年：

2024

DOI：

10.1007/s11655-024-3658-z