枇杷叶三萜酸联合重组人表皮生长因子对激素依赖性皮炎豚鼠皮肤屏障功能的修复及免疫失衡的影响
Effects of Loquat Leaf Triterpene Acid Combined with Recombinant Human Epidermal Growth Factor on Skin Barrier Function Repair and Immune Imbalance in Guinea Pigs with Hormone-dependent Dermatitis
姚莹 1张杰1
作者信息
- 1. 河南科技大学第二附属医院,河南洛阳 471000
- 折叠
摘要
目的 观察枇杷叶三萜酸(TAL)联合重组人表皮生长因子(rh-EGF)对激素依赖性皮炎(HDD)豚鼠模型皮肤屏障功能修复及免疫失衡的影响.方法 选取 40 只无特定病原(SPF)级豚鼠,随机分为对照组、模型组、rh-EGF组、TAL组和联合组,每组各8 只.除对照组外,其他各组涂抹 0.05%卤米松乳膏,连续 45 d,构建HDD模型.构建成功后,rh-EGF组涂抹rh-EGF凝胶,TAL组涂抹TAL溶液,联合组涂抹TAL和rh-EGF凝胶,对照组和模型组涂抹生理盐水,共15 d.记录各组临床症状并打分;检测经皮水分丢失(TEWL)、角质层含水量(WCSC)和皮脂量(SC);HE染色观察各组豚鼠皮肤组织病理变化;采用酶联免疫吸附测定(ELISA)法检测总免疫球蛋白E(IgE)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)水平.结果 与对照组比较,模型组症状明显、皮肤组织损坏严重,TEWL增加,WCSC和SC减少;免疫因子IgE和IL-4 水平升高、IFN-γ水平降低(P＜0.05).与模型组比较,rh-EGF组、TAL组和联合组用药后临床症状减轻、皮肤组织损伤不明显;TEWL减少,WCSC和SC增加,免疫因子IgE和IL-4 水平降低、IFN-γ水平升高(P＜0.05).且联合组与rh-EGF组和TAL组比较,作用效果更加明显(P＜0.05).TAL组和联合组各指标比较,差异无统计学意义(P>0.05).结论 TAL联合rh-EGF可有效缓解HDD豚鼠的临床症状,改善皮肤细胞损伤,修复皮肤屏障功能,提升免疫能力.
Abstract
Objective In order to observe the effects of triterpene acid of loquat(TAL)combined with recombinant human epidermal growth factor(rh-EGF)on the repair of skin barrier function and immune imbalance in guinea pigs models of hormone-dependent dermatitis(HDD).Methods Forty specified pathogen free(SPF)guinea pigs were selected and randomly divided into control group,model group,rh-EGF group,TAL group and combination group,with eight guinea pigs in each group.Except the control group,other groups were smeared with 0.05%halometasone cream for 45 d to construct HDD models.After the construction was successful,the rh-EGF group was smeared with rh-EGF gel,the TAL group was smeared with TAL solution,the combination group was smeared with TAL and rh-EGF gel,for 15 days.The clinical symptoms of each group were recorded and scored.Transepidermal water loss(TEWL),stratum corneum water content(WCSC),sebum volume(SC),hematoxylin-eosin(HE)staining were detected to observe the skin tissue morphology of guinea pigs in each group.The levels of total immunoglobulin E(IgE),interleukin-4(IL-4)and interferon-γ(IFN-γ)were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with the control group,the symptoms in the model group were obvious,and the skin tissue was severely damaged,TEWL increased,WCSC and SC decreased,immune factors IgE and IL-4 levels increased and IFN-γ level decreased(P＜0.05).Compared with the model group,the clinical symptoms in rh-EGF group,TAL group and combination group were relieved,and the skin tissue damage was not obvious,TEWL decreased,WCSC and SC increased,the levels of immune factors IgE and IL-4 decreased,and the level of IFN-γ increased(P＜0.05).Compared with the rh-EGF group and the TAL group,the effects of the combination group were more obvious(P＜0.05).There were no significant differences in the indexes between the TAL group and the combined group(P>0.05).Conclusion TAL combined with rh-EGF can effectively alleviate the clinical symptoms and skin cell damage of HDD guinea pigs,repair the skin barrier function of guinea pigs,and enhance immunity.
关键词
激素依赖性皮炎/枇杷叶三萜酸/重组人表皮生长因子/皮肤屏障功能/免疫失衡Key words
Hormone dependent dermatitis/Triterpene acid of loquat/Recombinant human epidermal growth factor/Skin barrier function/Immunity imbalance引用本文复制引用
基金项目
河南省科技发展计划(202102110258)
出版年
2024
国家科技期刊平台
NSTL
万方数据